Single Molecule/ Live Cell Imaging Facility
Welcome to our Imaging Facilities!!
Live Cell Imaging: Yokagawa Spinning Disc Confocal on a Nikon Ti system running Metamorph imaging software
Image shows a mammalian cell in early anaphase. The cell is expressing mCherry Tubulin which labels microtubules red and GFP-TPX2, a microtubule associated protein that binds to spindle fibers (yellow = green and red). At the lower left is a cell that is in telophase. the incipient daughter cells are connected by a midbody, a bundle of microtubules. TPX2 does not label the middle region of the midbody, only the flanking microtubules. 100x
Single Molecule Imaging: Custom-built PALM/TIRF system on a Nikon Ti system running Elements imaging software
Image shows a super resolution of microtubules taken with the new PALM system.
• Scheduled appointments for facility trained and approved users only
• Please contact Sai Keshavan Balchand (sbalchan@mcb.umass.edu) by email to schedule training and appointments for both systems
• Scheduling Calendar: http://calcium.physics.umass.edu/cgi-bin/RossLab.pl
Biology Department Video
