Welcome to our New Facilities!!

Live Cell Imaging: Yokagawa Spinning Disc Confocal on a Nikon Ti system running Metamorph imaging software

Image shows a mammalian cell in early anaphase. The cell is expressing mCherry Tubulin which labels microtubules red and GFP-TPX2, a microtubule associated protein that binds to spindle fibers (yellow = green and red). At the lower left is a cell that is in telophase. the incipient daughter cells are connected by a midbody, a bundle of microtubules. TPX2 does not label the middle region of the midbody, only the flanking microtubules. 100x

Single Molecule Imaging: Custom-built PALM/TIRF system on a Nikon Ti system running Elements imaging software

Image shows a density plot of a mammalian centrosome taken with the new PALM system. The color heatmap to the right indicates the numbers of molecules per site.

Technician: Stephanie Moeckel-Cole, PhD
• smcole@bio.umass.edu

• Scheduled appointments for facility trained and approved users only
• Please contact Technician by email to schedule training and appointments for both systems