Office: 436B Morrill IV South
B.S., The University of North Carolina at Chapel Hill, 1999
Ph.D., The University of California at Berkeley, 2005
The University of North Carolina at Chapel Hill, 2006-2010
We study the vital process of cell division. The goal of cell division is to ensure equal segregation of the genome, which is packaged into a defined number of chromosomes, between two daughter cells. Mistakes in this process lead to cells receiving an incorrect number of chromosomes – a state called aneuploidy. Since aneuploidy is the cause of Down syndrome and is linked to metastatic tumor progression, understanding cell division at its most basic levels has significant relevance to human disease and cancer.
In order to succeed at division, cells must coordinate a dizzying array of highly complex cellular events that include chromosome condensation, spindle assembly, and chromosome congression/alignment - to name a few (see Movie 1). On top of this, cells have evolved a biochemical pathway called the spindle assembly checkpoint (SAC) that acts as a surveillance mechanism to ensure that chromosome segregation does not occur prematurely. All of these processes are fair game for research in the Maresca lab but we are particularly focused on combining molecular and biochemical approaches with innovative high-resolution microscopy towards understanding SAC function and error correction mechanisms during mitosis.
The Spindle Assembly Checkpoint
The “brains” behind the SAC pathway reside on each chromosome at macro-molecular protein assemblies called kinetochores. Each kinetochore complex consists of at least 100 known proteins, which are present in multiple copy numbers and spatially organized into a specific and conserved molecular architecture. Kinetochores carry out two critical functions. First, they mediate the attachment of chromosomes to dynamic microtubules so they can be aligned and segregated by the spindle. When there are problems with this process, kinetochores fulfill their second essential function of producing an inhibitory signal that delays chromosome segregation when chromosomes are not properly aligned. We have previously discovered that a physical reorganization within each kinetochore structure, deemed intrakinetochore stretch, is associated with whether a “wait-anaphase” signal is generated (see Figure 1). We hypothesize that the kinetochore acts as a mechanical switch that functions upstream of checkpoint signaling proteins to determine whether a wait-anaphase signal is generated. We are using newly-emerging microscopy-based approaches to test this hypothesis.
Detecting and Correcting Improper Chromosome Attachments
Accurate segregation of the genetic material generally requires that every chromosome becomes bioriented with each of its sister kinetochores attached to microtubules emanating from opposite poles of the mitotic spindle. However, no cell is perfect, and during cell division chromosomes can become improperly attached. We are particularly interested in how cells detect and correct syntelic attachments - an aberrant attachment state in which both sister kinetochores are attached to the same pole (see Figure 2 ). Syntelic attachments trigger generation of the wait-anaphase signal in order to give the cell time to correct the error and biorient the chromosome before entering anaphase (see Movie 2 ). Understanding how the cell carries out detection and correction of syntelic attachments is of central importance because failure to do so leads to chromosome mis-segregation and aneuploidy (see Movie 3 ). We postulate that intrakinetochore stretch lies at the molecular intersection of both error detection and error correction mechanisms and plan to further investigate this theory experimentally.
Nachury, M.V., Maresca, T.J., Salmon, W.C., Waterman-Storer, C.M., Heald R., Weis, K. 2001. Importin beta is a mitotic target of the small GTPase Ran in spindle assembly. Cell, 104(1): 95-106.
Wignall, S.M., Deehan, R., Maresca, T.J., Heald, R. 2003. The condensin complex is required for proper spindle assembly and chromosome segregation in Xenopus egg extracts. The Journal of Cell Biology, 161(6): 1041-51.
Maresca, T.J., Freedman, B., Heald, R. 2005. Histone H1 is essential for mitotic chromosome architecture and segregation in Xenopus laevis egg extracts. The Journal of Cell Biology, 169(6): 859-69.
Maresca, T.J., Niederstrasser, H., Weis, K., Heald, R. 2005. Xnf7 contributes to spindle integrity through its microtubule-bundling activity. Current Biology, 15(19): 1755-61.
Yan, J., Maresca, T.J., Skoko, D., Heald, R., Marko, J.F. 2006. Micromanipulation studies of chromatin fibers in Xenopus egg extracts reveal ATP-dependent nucleosome assembly dynamics. Molecular Biology of the Cell, 18(2): 464-74.
Brown K.S.*, Blower M.D.*, Maresca T.J.* , Grammer T.C., Harland R.M., Heald R. 2007. Xenopus tropicalis egg extracts provide insight into scaling of the mitotic spindle. The Journal of Cell Biology, 176(6): 765-70.
*Indicates Co-first authorship
Gatlin, J.C., Matov, A., Groen, A.C., Needleman, D.J., Maresca, T.J., Danuser, G., Mitchison, T.J., Salmon, E.D. 2009. Spindle fusion requires dynein-mediated sliding of oppositely oriented microtubules. Current Biology, 19(4): 287-96.
Maresca, T.J., Salmon, E.D. 2009. Intrakinetochore stretch is associated with changes in kinetochore phosphorylation and spindle assembly checkpoint activity. The Journal of Cell Biology, 184(3): 373-81.
Groen, A.C.*, Maresca, T.J.*, Gatlin, J.C., Salmon, E.D., Mitchison, T.J. 2009. Functional overlap of microtubule assembly factors in chromatin-promoted spindle assembly. Molecular Biology of the Cell, 20(11): 2766-73.
*Indicates Co-first authorship
Maresca, T.J.*, Groen, A.C.*, Gatlin, J.C., Mitchison, T.J., Salmon, E.D. 2009. Spindle assembly in the absence of a RanGTP gradient requires localized CPC activity. Current Biology, 19(14): 1210-5.
*Indicates Co-first authorship
Needleman, D.J., Groen, A., Ohi, R., Maresca, T., Mirny, L., Mitchison, T. 2010. Fast Microtubule Dynamics in Meiotic Spindles Measured by Single Molecule Imaging: Evidence that the Spindle Environment does not Stabilize Microtubules. Molecular Biology of the Cell, 21(2): 323-33.
Maresca, T.J., Salmon, E.D. 2010. Welcome to a new kind of tension: Translating kinetochore mechanics into a wait anaphase signal. Journal of Cell Science,15;123(Pt 6): 825-35.
- Department at a Glance
- Current News
- Upcoming Events
- Job Openings
- How to Contact Us
- How to Find Us
- Administrative Services
- Computer Services
- Organization Chart/ Committee Structure
- Major Requirements
- Major Requirements Checklist (PDF)
- Recommended Course Sequences
- Integrative Experience
- Minor Requirements
- Departmental Honors
- Course Catalog
- Course Websites
- Course Applications
- Learning Goals
- Research Opportunities
- Awards & Scholarships
- BioSci Club
- Residential Programs
- Undergrad Handbook (PDF)
- Biology Computer Resource Center
- Central Microscopy
- Genomics & Bioinformatics
- Natural History Collections
- Single Molecule/Live Cell Imaging Facility
- Vibrating Probe Facility
- Zebrafish Facilities
- Make a Gift…