Table 1. The Arabidopsis
thaliana YSL family of
proteins
|
|
Progress in Walker Lab |
|||||||
|
Name (length) |
Chromosome based name |
A. thaliana ESTs in database |
Similarity to Maize YS1 (682 aa) |
Full Length cDNA |
Yeast functional complementation |
Confirmed T-DNA Insertion(s) |
Phenotype |
Localization by GFP (stable transformants; native promoter) |
|
YSL1 (673 aa) |
At4g24120 |
701496573 (Ohio State clone set) 161F15T7 161F15SP (Lambda-PRL2 mixed) 119D15T7 (Lambda-PRL2 mixed) 109C6T7 (Lambda-PRL2 mixed) R15661 (RIKEN FULL LENGTH) |
Identities 399/665
(60%) Positives 497/665 (74%) |
YES
|
transports Fe(II)-NA (complementation of yeast fet3fet4 mutant) |
ysl1-1 (SALK_016287) mRNA detected by RT-PCR ysl1-2 (SALK_034534) null (by RT-PCR) ysl1-3 (SALK_051348) null (by RT-PCR) |
Not detected |
|
|
YSL2 (664 aa) |
At5g24380 |
RZL17e03F (Columbia roots) E6H10T7 (CD4-13 3D hypocotyl) TAP0088 (AC16H cell suspension culture) |
Identities 408/658
(62%) Positives 517/658 (78%) |
YES |
transports
Fe(II)-NA (complementation of yeast fet3fet4 mutant) transports Cu-NA (complementation of yeast ctr1 mutant) |
ysl2-1 (SALK_001348) null (by RT-PCR) |
Lateral plasma membrane of xylem parenchyma |
|
|
YSL3 (675 aa) |
At5g53550 |
none |
Identities 411/668
(61%) Positives 516/668 (76%) |
YES
(ELW gene-specific RT-PCR of leaves, and direct cloning into yeast since cannot be cloned intact in E. coli) |
In progress |
ysl3-1 (SALK_064683) null (by RT-PCR) |
Plasma membrane of xylem parenchyma |
|
|
YSL4 (670 aa) |
At5g41000 |
none |
Identities 333/644
(51%) Positives 452/644 (69%) |
YES
|
transports Fe(II)-NA (complementation of yeast fet3fet4 mutant) |
SALK_006995
false—no T-DNA at locus ysl4-2
(SALK_025447) under
investigation |
In
progress |
No data |
|
YSL5 (714 aa) |
At3g17650 |
APZ20g12R APZ20g12F (2-6 week above ground) 186N21XP 186N21T7 (Lambda-PRL2 mixed) G2G7T7 (CD4-13 3D hypocotyl) R14582 (RIKEN FULL LENGTH) |
Identities 345/680
(50%) Positives 463/680 (67%) |
YES
|
transports
Fe(II)-NA (complementation of yeast fet3fet4 mutant) transports Cu-NA (complementation of yeast ctr1 mutant) |
ysl5-1 (SALK_058656) null (by RT-PCR) |
Single mutants have no obvious phenotypes |
No data |
|
YSL6 (676 aa) |
At3g27020 |
701549206 (Col-0, inflorescence-2) APD47h09_f (2-6 week above ground) 77B5T7 (Lambda-PRL2 mixed) 117N21T7 (Lambda-PRL2 mixed) 163G5T7 (Lambda-PRL2 mixed) SQ120d04F (green siliques) 282D4T7 (Lambda-PRL2 mixed) Ceres_34773 (Ceres FULL LENGTH) |
Identities 349/646
(54%) Positives 461/646 (71%) |
YES
|
transports
Fe(II)-NA (complementation of yeast fet3fet4 mutant) |
ysl6-1 (SALK_015850) under investigation ysl6-2 (SALK_015853 ysl6-4 (SALK_119560) under investigation ysl6-5 (SALK_093392) null (by RT-PCR) |
in progress |
|
|
YSL7 (688 aa) |
At1g65730 |
(developing seed FULL LENGTH) |
Identities 341/674
(50%) Positives 472/674 (69%) |
EST
600035805R1 |
transports Fe(II)-NA (complementation of yeast fet3fet4 mutant) |
ysl7-1 (SALK_014212) under investigation |
No data |
|
|
YSL8 (724 aa) |
At1g48370 |
R12830 (RIKEN FULL LENGTH) |
Identities 337/672
(50%) Positives 454/672 (67%) |
YES
|
transports Fe(II)-NA (complementation of yeast fet3fet4 mutant) |
ysl8-1 (SALK_006393) mRNA present (by RT-PCR) ysl8-2 (SALK_000907) mRNA very low or absent by (RT-PCR) |
Single mutants have no obvious phenotypes |
Not detected |