of axons and
in the zebrafish forebrain.
Red cells are a glial "bridge" that spans the midline
prior to axon crossing. Purple square indicates the
region shown in the two movies below. The movies on the right show frontal views of the the entire zebrafish head, eyes on either side.
|30 hours: RGC axons near
midline: Confocal movie of axons (green) and GFAP positive glial
cells (red) at 30 hrs. Retinal axons have just grown into the
brain. Click to see 3D movie of this image. Movie by M.
|3D rotating image of glial cells
commissural axons (bottom green band) and retnial axons
(top greeen band) in the diencephalon. Click on the image
to see a rapidly rotating 3D movie of this data set.
Deconvolution software (Openlab) was used to improve the
image, allowing us to view details of glial cell morphology
not otherwise visible. Movie by M. Barresi.
|48 hours: A well formed chiasm. Confocal movie of axons (green) and GFAP positive glial cells (red) at 48 hrs. At this age, retinal axons have crossed the midlien to form the optic chiasm. Click to see 3D movie of this image.Movie by M. Barresi|
|Confocal movie showing
commissural axons (red) growing in
relation to cells expressing a Sema3D-GFP fusion protein
(green) under the control of an HSP70, heat-shock inducible, promoter. Embryos were injected with an HSP70-Sema3D-GFP plasmid construct at the 2 cell stage to produce an embryo with mosaic inheritence of the plasmid. Embryos were grown to 19 hours, heat-shocked for 50 minutes to induce Sema3D-GFP
expression, then grown to 24 hours when they were fixed
and labeled with an antibody to show growing axons (red).
These images were acquired on a Zeiss meta confocal microscope by Anandita Seth in the Karlstrom Lab. Movie by A. Seth
|48 hours: A well formed chiasm. Confocal movie ofZn-5 labeled retinal ganglion cells and axons (green) at 48 hrs. Retinal axons have just grown into the brain. Zn-5 also labels the adenohypophysis (pituitary placode). Click to see 3D movie of this image. Movie by M. Barresi|