The modified culture media allowed the gregarine sporadins to live for at least five days. By five days, however, bacteria and fungus were overgrown in the Petri dishes, and this impeded the gregarine movement. We designed our experiments to conclude within 24 hours.
Isoelectric point focusing produced a pH gradient from 4-12 over a distance of six centimeters in the trough. Motion of the gregarines was recorded to AVI files and velocities analyzed, Fig. 5.