Conclusions
The modified culture media was adequate for keeping gregarines in vitro for the duration of most experiments. Typically, experiments were designed for one day.
Although there is no obvious optimum pH, gregarines could live in a wide pH range, and suggests pH association with motility (Fig. 5).
- It is uncertain whether the sporadin are moving away or towards a certain pH, or whether there are optimal proton levels for movement at pH 8.
- The gregarines were motionless at the extreme ends of the gradient - dying and bursting at the highest pH end, but retaining their shape at the lowest pH.
The integration of both hardware and software in the MRMS was successfully applied in this experiment, and can be used as a foundation for a broad range of applications, such as immunology, oncology, and embryology.