@text Kunkel's construction kit for plotting output of Non-Invasive Vector data relative to a source outline- Data were collected with the program VPW, developed Eric Karplus. This data was collected at the University of Massachusetts Vibrating Probe Facility (UMASS-VPF), at Amherst by Joe Kunkel using rig#1 on June 24, 1996. Electrode Puller: Flaming Brown Microelectrode Puller, Program 8. Silanization: 250C baking with Sigma SVPF Rig #1 Equipment: Zeis IM35 microscope. NVPF Custom 3-D Stepper motor system. UMASS VPF Customized Faraday Cage with steel plate + rubber inner tube vibration suppession. Applicable Electronics Model 300 Dual Ion Probe Amplifier. Applicable Electronics Microstepper Controller. Two Kinemages of a double artificial proton gradient scan under oil. Chose the Kinemage using the KINEMAGE pulldown menu. Kinemage 1 was recorded at a lower resolution with 80 um steps. Kinemage 2 was recorded at higher resolution with 40 um steps. Scans 1 & 2: Scan 7x22 grid at 80 um steps. The raw dVx,dVy,dVz proton microvolt data was multiplied by 0.1 0.1 0.1 and added to the location. This depended on the original 3D proton sampling rules collecting 3 3-D observations within a 40 sec/location grid sampling scheme and used the median of each dimension for plotting. The animate feature is turned on and several views are available. The original 640x480 8bit image of the diffusion source and H-electrode of a similar setup is available as a downsized JPEG format (320x240) h60613.jpg at our anonymous FTP site accessible via the WWW site: http://www.bio.umass.edu/biology/kunkel/ 2) The different views have different magnifications and centers. Try them. 3) Turn on the Stereo feature under the Other pull-down menu and view the data in stereo pairs while moving it on the screen! Notice that you can envision the source of the ions by extrapolating the vectors back to the follicle surface. @kinemage 1 @caption Artificial Diffusion Source: Two Proton sources. 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points} @group {view 7} dominant animate moview= 7 @vectorlist {vectors} @vectorlist {ovariole} instance= {center} @vectorlist {scan points} @group {view 8} dominant animate moview= 8 @vectorlist {vectors} @vectorlist {ovariole} instance= {center} @vectorlist {scan points} @group {view 9} dominant animate moview= 9 @vectorlist {vectors} @vectorlist {ovariole} instance= {center} @vectorlist {scan points} @kinemage 2 @caption Artificial Diffusion Source: Two Proton sources. 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