@text Kunkel's construction kit for plotting output of Non-Invasive Vector data relative to a source outline- Data were collected with the program 3DVIS, developed at the National Vibrating Probe Facility, Marine Biology Laboratory, Woods Hole, MA. This data was collected at the Satellite Vibrating Probe Facility (SVPF), U. Massachusetts at Amherst by Joe Kunkel and Ellen Faszewski using SVPF rig#2 on March 6, 1996. Electrode Puller: NVPF Flaming Brown Microelectrode Puller. Silanization: 250C baking with Sigma SVPF Rig #2 Equipment: Zeis IM35 microscope. NVPF Custom 3-D Stepper motor system. SVPF Customized Faraday Cage with steel plate + rubber grommet vibration suppession. Applicable Electronics Model 300 Dual Ion Probe Amplifier. Applicable Electronics Microstepper Controller. Two Kinemages of the same type of data set from Kunkel & Faszewski Biol. Bull. (1995) A Periplaneta americana follicle was scanned twice with a Proton Specific Electrode, once at 32 um intervals and second, with an hour delay, at a 40 um intervals. Kinemage 1: Scan A at 32 um steps. The raw dVx,dVy,dVz proton microvolt data was multiplied by -1 1 1 and added to the location. The animate feature is not implemented in this scan. Kinemage 2: Scan B at 40 um steps. The raw dVx,dVy,dVz proton microvolt data was multiplied by -1 1 1 and added to the location. The animate feature is turned on and several views are available. 1) The oocyte outline was computed in a spreadsheet from data extracted from an image grabbed at the time of the data scanning. The original 640x480 8bit image of the oocyte is available as a downsized JPEG format (320x240) pa60306.jpg at our anonymous FTP site accessable via the WWW site: http://www.bio.umass.edu/biology/kunkel/ 2) The different views have different magnifications and centers. Try them. 3) Turn on the Stereo feature under the Other pull-down menu and view the data in stereo pairs while moving it on the screen! Notice that you can envision the source of the ions by extrapolating the vectors back to the follicle surface. @kinemage 1 @caption Cockroach oocyte Pa60306a data. 1x vectors using -1 1 1 multiplier. @perspective @thinline @zoom 1.0 @zslab 200 @center 0.0 0.0 0.0 @matrix 0.707 0.0 0.707 0.0 1.0 0.0 -0.707 0.0 0.707 @2zslab 350 @2center 0.0 0.0 0.0 @2matrix 0.707 0.0 0.707 0.0 1.0 0.0 -0.707 0.0 0.707 @3zoom 1.0 @3zslab 350 @3center 0.0 0.0 0.0 @3matrix 0.707 0.0 0.707 0.0 1.0 0.0 -0.707 0.0 0.707 @4zoom 1.0 @4zslab 350 @4center 0.0 0.0 0.0 @4matrix 0.707 0.0 0.707 0.0 1.0 0.0 -0.707 0.0 0.707 @5zoom 1.0 @5zslab 350 @5center 0.0 0.0 0.0 @5matrix 1.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 1.0 @6zoom 1.58 @6zslab 350 @6center 0.0 0.0 0.0 @6matrix 1.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 1.0 @7zoom 0.75 @7zslab 240 @7center 800.0 100.0 0.0 @7matrix 1.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 1.0 @group {Labels} nobutton @labellist {Drawn} color= white master= {Labels} {100 um} 90 180 0 @group dominant {calibration} on 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26.45 0 -289 29.9 0 -322 33.35 0 -350 35.65 0 -371 39.1 0 -391 40.25 0 -397 41.4 0 @kinemage 2 @caption Cockroach oocyte Pa60306b 1x data using -1 1 1 multiplier. 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dominant animate moview= 1 @vectorlist {vectors} @vectorlist {ovariole} instance= {center} @vectorlist {scan points} @group {view 2} dominant animate moview= 2 @vectorlist {vectors} @vectorlist {ovariole} instance= {center} @vectorlist {scan points} @group {view 3} dominant animate moview= 3 @vectorlist {vectors} @vectorlist {ovariole} instance= {center} @vectorlist {scan points} @group {view 4} dominant animate moview= 4 @vectorlist {vectors} @vectorlist {ovariole} instance= {center} @vectorlist {scan points} @group {view 5} dominant animate moview= 5 @vectorlist {vectors} @vectorlist {ovariole} instance= {center} @vectorlist {scan points} @group {view 6} dominant animate moview= 6 @vectorlist {vectors} @vectorlist {ovariole} instance= {center} @vectorlist {scan points} @group {view 7} dominant animate moview= 7 @vectorlist {vectors} @vectorlist {ovariole} instance= {center} @vectorlist {scan points} @group {view 8} dominant animate moview= 8 @vectorlist {vectors} @vectorlist {ovariole} instance= {center} @vectorlist {scan points} @group {view 9} dominant animate moview= 9 @vectorlist {vectors} @vectorlist {ovariole} instance= {center} @vectorlist {scan points}