Differentiated murine myoblasts

Glenn, H. L.

Differentiated murine myoblasts

Murine myoblasts, C2C12 cells, were cultured in low serum differentiation medium for 3 days then fixed and tripled labeled for actin (red) with rhodamine-phalloidin; muscle-specific myosin heavy chain (green) by immuonfluorescence with Alexafluor 488 secondary antibody; and nuclei (blue) with To-Pro-3. As myoblasts differentiate, they upregulate myosin and other muscle-specific proteins, and fuse to form multinucleate myotubes, which mature into muscle fibers.

Cells were imaged with a Nikon C1 scanning confocal system with a Nikon TE 2000 inverted microscope using a 40x oil immersion objective lens (N.A. 1.3). Images were processed with the C1 EZ-Viewer software.