Fixative stock: 37% Formaldehyde: small bottle under chemical hood in prep lab.
TritonX-100: 10% stock, in hood in prep lab
To make 10 mL of fixative:
Make in fume hood
To fix the cells:
remove PBS, replace with fresh PBS, remove again, replace). Leave
cells in this PBS—until you add the fixative).
Important things to remember
Wear gloves and be very careful of the fixatives!!!
Remember which side of the coverslip has the cells.
Discard used fixative into the appropriate hazardous waste container in
the chemical hood in the main lab.
32% Paraformaldehyde: bottle under chemical hood in main lab.
Detergent: 10% triton X 100
To make 10 mls of fixative:
Paraformaldehyde: 1 mL stock
10% triton: 0.5 ml
10X PBS -/- 1 mL
add water make 10 mL (8.5 mL)
Use same method as Formaldehyde fixation.