Splitting Mammalian Tissue Culture Cells
(this is conditioned medium).
1 ml conditioned medium (supernatant)
1 ml of cell suspension
3 mls of fresh media
To make ConA coverslips: Product is C-2631 from sigma
Made it like this: 100mgs, want 0.5 mg/ml. Add 200 mls water, syringe filter. Store at -20 in aliquots.
See my lab book Jan 2006, page 50.
To make medium for growth of drosphila cells: