Project Description :
The Karlstrom Lab investigates the formation and growth of the ventral forebrain and pituitary gland in the zebrafish. We are now investigating how cell-cell signaling mediated by a small protein called “Hedgehog” (Hh) controls stem cell proliferation to regulate tissue growth and cell renewal in larvae and adults. This position is for students wishing to pursue serious honors thesis research, including summer research. The student will work to characterize zebrafish transgenic lines that allow the conditional (time and tissue specific) regulation of Hh signaling in zebrafish. These lines will then be used to understand how Hh signaling affects growth and cell renewal in the forebrain through larval stages and in the adult. A wide range of techniques will be employed in this project, including immunohistochemistry, in situ hybridization, PCR and DNA work, and confocal microscopy.