Cynthia L. Baldwin

Professor of Veterinary and Animal Sciences, University of Massachusetts

Email: cbaldwin@vasci.umass.edu
C. Baldwin Vet & Animal Sciences Web Site

Ph.D.: Cornell University
Postdoctoral Training: International Laboratory for Research on Animal Diseases, Nairobi, Kenya

Cellular Immunity: Intracellular Microbes and gamma delta T cells

While macrophages are normally considered to be a primary cell for mediating innate immune responses by phagocytosing and destroying microbes that infect mammals, some microbial organisms including bacteria and protozoa have specifically adapted themselves to survive and replicate in host cells thereby "hiding" from protective immune responses. We study Brucella abortus, a bacterium that causes disease in a wide variety of agricultural animals and in people, to evaluate how some animals but not others successfully prevent infections by intracellular pathogens. We are particularly interested in the role of host cytokines such as interleukins and gamma-interferon produced by T lymphocytes in controlling the infection through their ability to activate macrophages for antimicrobial activities. In addition we are interested in identifying genes of the bacteria that facilitate their survival in host macrophages thus allowing them to establish chronic infections.

Our second major area of research evaluates gamma delta T cells, a subpopulation of T lymphocytes that comprise a major proportion of lymphocytes in mammals but whose role in mediating protective immunity is unclear at this time. We are studying how this subpopulation of T lymphocytes is activated by constitutively expressed molecules on macrophages and the ability of a killed leptospira vaccine to prime gamma delta T cells for recall responses to leptospira components. We are also interested in the role a lineage-specific cell-surface molecule of gamma delta T cells, known as WC1, plays in signal transduction and activation of these cells and understanding why expression of particular isoforms of WC1 correlates with functional differences of the T cells.

Representative publications:

McGill JL, Sacco RE, Baldwin CL, Telfer JC, Palmer MV, Waters RW.  2014.  Specific Recognition of Mycobacterial Protein and Peptide Antigens by γδ T Cell Subsets following Infection with Virulent Mycobacterium bovis.. J Immunol.

Kambayashi T, Behrens EM, Bachmann MH, Salicioni AM, Baldwin CL, Karimi MA, Lee E.  2014.  Measuring cytotoxicity by bioluminescence imaging outperforms the standard chromium-51 release assay.. PlosOne. 10.1371/journal.pone.0089357

Chen C, Herzig CTA, Alexander LJ, Keele JW, McDaneld TG, Telfer JC, Baldwin CL.  2012.  Gene number determination and genetic polymorphism of the gamma delta T cell co-receptor WC1 genes.. BMC Genet. 13:86.

Goenka R, Guirnalda PD, Black SJ, Baldwin CL.  2012.  B Lymphocytes provide an infection niche for intracellular bacterium Brucella abortus.. J Infect Dis. 206(1):91-8.

Hudgens E, Tompkins D, Boyd P, Lunney JK, Horohov D, Baldwin CL.  2011.  Expressed gene sequence of the IFNγ-response chemokine CXCL9 of cattle, horses, and swine.. Veterinary immunology and immunopathology. 141(3-4):317-21.

Wang F, Herzig CTA, Chen C, Hsu H, Baldwin CL, Telfer JC.  2011.  Scavenger receptor WC1 contributes to the γδ T cell response to Leptospira.. Molecular immunology. 48(6-7):801-9.

Goenka R, Parent MA, Elzer PH, Baldwin CL.  2011.  B cell-deficient mice display markedly enhanced resistance to the intracellular bacterium Brucella abortus.. The Journal of infectious diseases. 203(8):1136-46.

Herzig CTA, Waters RW, Baldwin CL, Telfer JC.  2010.  Evolution of the CD163 family and its relationship to the bovine gamma delta T cell co-receptor WC1.. BMC evolutionary biology. 10:181.

Herzig CTA, Lefranc M-P, Baldwin CL.  2010.  Annotation and classification of the bovine T cell receptor delta genes.. BMC genomics. 11:100.

Tompkins D, Hudgens E, Horohov D, Baldwin CL.  2010.  Expressed gene sequences of the equine cytokines interleukin-17 and interleukin-23.. Veterinary immunology and immunopathology. 133(2-4):309-13

Wang F, Herzig C, Ozer D, Baldwin CL, Telfer JC.  2009.  Tyrosine phosphorylation of scavenger receptor cysteine-rich WC1 is required for the WC1-mediated potentiation of TCR-induced T-cell proliferation.. European journal of immunology. 39(1):254-66.

Chen C, Herzig CTA, Telfer JC, Baldwin CL.  2009.  Antigenic basis of diversity in the gammadelta T cell co-receptor WC1 family.. Molecular immunology. 46(13):2565-75.

Chen C, Herzig CTA, Baldwin CL.  2009.  Expressed gene sequence of bovine IL23A and IL23R.. Veterinary immunology and immunopathology. 128(4):425-30.

Herzig CTA, Baldwin CL.  2009.  Genomic organization and classification of the bovine WC1 genes and expression by peripheral blood gamma delta T cells.. BMC genomics. 10:191.

Blumerman SL, Herzig CTA, Wang F, Coussens PM, Baldwin CL.  2007.  Comparison of gene expression by co-cultured WC1+ gammadelta and CD4+ alphabeta T cells exhibiting a recall response to bacterial antigen.. Molecular immunology. 44(8):2023-35.

Blumerman SL, Wang F, Herzig CTA, Baldwin CL.  2007.  Molecular cloning of bovine chemokine receptors and expression by WC1+ gammadelta T cells.. Developmental and comparative immunology. 31(1):87-102.

Parent MA, Goenka R, Murphy E, Levier K, Carreiro N, Golding B, Ferguson G, Roop MR, Walker GC, Baldwin CL.  2007.  Brucella abortus bacA mutant induces greater pro-inflammatory cytokines than the wild-type parent strain.. Microbes and infection / Institut Pasteur. 9(1):55-62.

Blumerman SL, Herzig CTA, Baldwin CL.  2007.  WC1+ gammadelta T cell memory population is induced by killed bacterial vaccine.. European journal of immunology. 37(5):1204-16.

Herzig CTA, Blumerman SL, Baldwin CL.  2006.  Identification of three new bovine T-cell receptor delta variable gene subgroups expressed by peripheral blood T cells.. Immunogenetics. 58(9):746-57.

Blumerman SL, Herzig CTA, Rogers AN, Telfer JC, Baldwin CL.  2006.  Differential TCR gene usage between WC1- and WC1+ ruminant gammadelta T cell subpopulations including those responding to bacterial antigen.. Immunogenetics. 58(8):680-92.

Rogers AN, Vanburen DG, Zou B, Lahmers KK, Herzig CTA, Brown WC, Telfer JC, Baldwin CL.  2006.  Characterization of WC1 co-receptors on functionally distinct subpopulations of ruminant gamma delta T cells.. Cellular immunology. 239(2):151-61.

Sathiyaseelan J, Goenka R, Parent M, Benson RM, Murphy EA, Fernandes DM, Foulkes AS, Baldwin CL.  2006.  Treatment of Brucella-susceptible mice with IL-12 increases primary and secondary immunity.. Cellular immunology. 243(1):1-9

Baldwin, C.L. and R. Goenka. (2006) Host Immune responses to the intracellular bacteria Brucella : does the bacteria instruct the host to facilitate chronic infection? Critical Reviews in Immunology, in press.

Herzig, C.T.A., S.L. Blumerman and C.L. Baldwin. (2006) Identification of three new bovine T cell receptor delta variable gene subgroups expressed by peripheral blood T cells. Immunogenetics, in press.

Blumerman, S., C. Herzig, F. Wang, P. Coussens, and C. Baldwin. Comparison of gene expression by co-cultured WC1 + g d and CD4 + a b T cells exhibiting a recall response to bacterial antigen. Molecular Immunology, in press.

Herzig, C., S. Blumerman, M-P. Lefranc and C. Baldwin. (2006) Bovine T cell receptor gamma variable and constant genes: combinatorial usage by circulating ?d T cells. Immunogenetics 58:138-151.

Rogers, A.N., D. Van Buren, B. Zou, K. Lahmers, C. Herzig, W.C. Brown, J.C. Telfer and C.L. Baldwin. (2006) Characterization of WC1 co-receptors on functionally distinct subpopulations of ruminant ?d T cells. Cell. Immunol. 239:151-161.

Blumerman, S., C. Herzig, A. Rogers and C.L. Baldwin. (2006) Differential TCR gene usage between WC1 - and WC1 + ruminant ?d T cell subpopulations including those responding to bacterial antigen. Immunogenetics 58:680-692.

Rogers, A ., D. G. VanBuren, E.E. Hedblom, M.E. Tilahun, J.C. Telfer and C.L. Baldwin. (2005) g d T cell function varies with the expressed WC1 coreceptor. J. Immunol. 174:3386-3393.

Parent M., Bellaire B., Murphy E.A., Roop R.M., and C.L. Baldwin (2002) Role of Brucella abortus siderophore 2,3-dihydroxybenzoic acid (DHBA) for survival in macrophages, trophoblasts and in vivo. Microbial Pathogenesis 32:239-248.
Sathiyaseelan, T., B. Naiman, S. Welte, N. Machugh, S. J. Black and C. L. Baldwin (2002). "Immunological characterization of a gammadelta T-cell stimulatory ligand on autologous monocytes." Immunology 105(2): 181-9.

Murphy, E. A., J. Sathiyaseelan, M. A. Parent, B. Zou and C. L. Baldwin (2001). "Interferon-gamma is crucial for surviving a Brucella abortus infection in both resistant C57BL/6 and susceptible BALB/c mice." Immunology 103(4): 511-8.

Naiman, B. M., D. Alt, C. A. Bolin, R. Zuerner and C. L. Baldwin (2001). "Protective killed Leptospira borgpetersenii vaccine induces potent Th1 immunity comprising responses by CD4 and gammadelta T lymphocytes." Infect Immun 69(12): 7550-8.

Sathiyaseelan, J., X. Jiang and C. L. Baldwin (2000). "Growth of Brucella abortus in macrophages from resistant and susceptible mouse strains." Clin Exp Immunol 121(2): 289-94.