John M. Clark

Professor of Veterinary and Animal Sciences, University of Massachusetts

Email: jclark@vasci.umass.edu
J. Clark Vet and Animal Sciences Web Site

Ph.D.: Michigan State University
Postdoctoral Training: Marine Biology Laboratory, Woods Hole, Mass.
Honors: Rohm & Haas Recognition Award in Insect Physiology, Biochemistry and Toxicology, ESA, Eastern Branch, 1997

Molecular Action of Pesticides and Cellular Basis for Resistance

My research program addresses a number of issues broadly defined as pesticide toxicology. In our attempts to evaluate the toxic action of pesticides on target organisms, we have elucidated many fundamental physiological processes and recently began the study of these interactions at the molecular level. Given the effectiveness and widespread use of pesticides, it was probably naive not to have envisioned the environmental concerns that the use of these chemicals has caused. Nevertheless, the negative aspects of pesticides and their use have resulted in a much more complete and rigorous understanding of how they locate and bind target molecules, their environmental fate and degradation, and problems associated with extensive overuse, such as resistance. Thus, the most enduring contribution of pesticides such as DDT and the cyclodienes may be in providing well-studied models to assess the advantages or disadvantages of new molecular biotechnologies as they become available for use in pesticide science.

The focus of my research effort is on the efficacious and environmentally sound use of pesticides. Specifically, my research team is interested in the molecular mechanisms of pesticide action on target and non-target organisms, xenobiotic metabolism of pesticides and molecular mechanisms of pesticide resistance. We examine the effects of pesticides in a variety of vertebrate and invertebrate systems and currently use molecular biological techniques to detect mutations.

Representative publications:

Clark JM, Symington SB.  2012.  Advances in the mode of action of pyrethroids.. Topics in current chemistry. 314:49-72.

Kim JH, Min JS, Kang JS, Kwon DH, Yoon KS, Strycharz J, Koh YH, Pittendrigh BR, Clark JM, Lee SH.  2011.  Comparison of the humoral and cellular immune responses between body and head lice following bacterial challenge.. Insect biochemistry and molecular biology. 41(5):332-9.

Pittendrigh BR, Berenbaum MR, Seufferheld MJ, Margam VM, Strycharz JP, Yoon KS, Sun W, Reenan R, Lee SH, Clark JM.  2011.  Simplify, simplify: Lifestyle and compact genome of the body louse provide a unique functional genomics opportunity.. Communicative & integrative biology. 4(2):188-91.

Strycharz JP, Berge NM, Alves A-M, Clark JM.  2011.  Ivermectin acts as a posteclosion nymphicide by reducing blood feeding of human head lice (Anoplura: Pediculidae) that hatched from treated eggs.. Journal of medical entomology. 48(6):1174-82.

Yoon KS, Strycharz JP, Baek JH, Sun W, Kim JH, Kang JS, Pittendrigh BR, Lee SH, Clark JM.  2011.  Brief exposures of human body lice to sublethal amounts of ivermectin over-transcribes detoxification genes involved in tolerance.. Insect molecular biology. 20(6):687-99.

Kirkness EF, Haas BJ, Sun W, Braig HR, Perotti AM, Clark JM, Lee SH, Robertson HM, Kennedy RC, Elhaik E et al..  2010.  Genome sequences of the human body louse and its primary endosymbiont provide insights into the permanent parasitic lifestyle.. Proceedings of the National Academy of Sciences of the United States of America. 107(27):12168-73.

Hodgdon HE, Yoon KS, Previte DJ, Kim HJ, Aboelghar GE, Lee SH, Clark JM.  2010.  Determination of knockdown resistance allele frequencies in global human head louse populations using the serial invasive signal amplification reaction.. Pest management science. 66(9):1031-40.

Clark JM.  2009.  Determination, mechanism and monitoring of knockdown resistance in permethrin-resistant human head lice, Pediculus humanus capitis.. Journal of Asia-Pacific entomology. 12(1):1-7.

Clark JM, Symington SB.  2008.  Neurotoxic implications of the agonistic action of CS-syndrome pyrethroids on the N-type Ca(v)2.2 calcium channel.. Pest management science. 64(6):628-38.

Putnam RA, Doherty JJ, Clark JM.  2008.  Golfer exposure to chlorpyrifos and carbaryl following application to turfgrass.. Journal of agricultural and food chemistry. 56(15):6616-22.

Strycharz JP, Yoon KS, Clark JM.  2008.  A new ivermectin formulation topically kills permethrin-resistant human head lice (Anoplura: Pediculidae).. Journal of medical entomology. 45(1):75-81.

Yoon KS, Kwon DH, Strycharz JP, Hollingsworth CS, Lee SH, Clark JM.  2008.  Biochemical and molecular analysis of deltamethrin resistance in the common bed bug (Hemiptera: Cimicidae).. Journal of medical entomology. 45(6):1092-101.

Clark JM, Symington SB.  2007.  Pyrethroid action on calcium channels: neurotoxicological implications.. Invertebrate neuroscience : IN. 7(1):3-16.

Qiu L, Gao J-R, Clark JM.  2005.  Sequencing and characterization of a cDNA encoding a ferritin subunit of Colorado potato beetle, Leptinotarsa decemlineata.. Archives of insect biochemistry and physiology. 60(3):140-50.

Gerade BB, Lee SH, Scott TW, Edman JD, Harrington LC, Kitthawee S, Jones JW, Clark JM.  2004.  Field validation of Aedes aegypti (Diptera: Culicidae) age estimation by analysis of cuticular hydrocarbons.. Journal of medical entomology. 41(2):231-8.

Yoon KS, Gao J-R, Lee SH, Clark JM, Brown L, Taplin D.  2003.  Permethrin-resistant human head lice, Pediculus capitis, and their treatment.. Archives of dermatology. 139(8):994-1000.

Putnam RA, Nelson JO, Clark JM.  2003.  The persistence and degradation of chlorothalonil and chlorpyrifos in a cranberry bog.. Journal of agricultural and food chemistry. 51(1):170-6.

Soderlund D.M., Clark J.M., Sheets L.P., Mullin L.S., Piccirillo V.J., Sargent D., Stevens J.T., and M.L. Weiner (2002) Mechanisms of pyrethroid neurotoxicity: implications for cumulative risk assessment. Toxicology 171:3-59

Putnam R.A., and J.M. Clark (2002) Persistence and Degradation of Chlorothalonil and Chlorpyrifos in a Cranberry Bog. J. Agric. & Food Sci. (in press).

Yoon K.S., and J.M. Clark (2002) Selective Induction of Abamectin Metabolism by Dexamthasone, 3-methylcholanthrene and Phenobartital in Colorado Potato Beetle, Leptinotarsa decemineata (Say). Pestic. Biochem. Physiol. 73: 74-86.

Haith D.A., Lee P.O., Clark J.M. Roy G.R. and M.J Imboden (2002) Modeling Pesticide Volatilization from Turf. J. Env. Qual. 31: 724-729.

Clark J.M., Lee S.H., Kim H.J. Yoon K.S. and A. Zhang (2001) DNA-based Genotyping Techniques for the Detection of Point Mutations associated with Insecticide Resistance in Colorado Potato Beetle. Pest Management Sci. 57: 1-7.

Lee, S.H., Yoon, K.S., Williamson, MS, Goodson, S.J., Takano-Lee, M., Edman, J.D., Devonshire, A.L. and Clark, J.M. (2000) Molecular Analysis of kdr-like Resistance in Permethrin-Resistant Strains of Head Lice, Pediculosis capitis. Pestic. Biochem. Physiol. 66:130-143.

Zhang, A., Dunn, J.B. and Clark, J.M. (1999) An Efficient Strategy for Validation of a Point Mutation associated with Acetylcholinesterase Sensitivity to Azinphosmethyl in Colorado Potato Beetle. Pestic. Biochem. Physiol. 64: 120-28.

Lee, S.H., Dunn, J.B., Clark, J.M. and Soderlund, D.M. (1999). Molecular Analysis of kdr-like Resistance in a Permethrin-Resistant Strain of Colorado Potato Beetle. Pestic. Biochem. Physiol. 63:63-75.

Tessier, D. M. and Clark, J. M. (1999) Hapten Design in the Development of Competitive Enzyme-Linked Immunosorbent Assays for Genotoxic Metabolites of Alachlor. J. Agric. Food Chem. 47: 3925-3933.

Clark, J.M. (1997) Insecticides as tools in probing vital receptors and enzymes in the nervous system. Review Article: In: Crop Protection Chemicals: Tools for Life Sciences (F. Matsumura, ed.) Pestic. Biochem. Physiol. 57, 225-254.

Beeman, R.W., Thomson, M.S., Clark, J.M., DeCamillis, M.A. and Denell, R.E. (1996) Woot, an active, gypsy-class retrotransposon in the flour beetle, Tribolium castaneum, is associated with a recent mutation. Genetics 143 (1), 417-426.

Zhu, K.Y. and Clark, J.M. (1996) A point mutation of acetylcholinesterase associated with azinphosmethyl resistance and reduced fitness in Colorado potato beetle. Pestic. Biochem. Physiol. 55, 100-108.

Zhu, K.Y. and Clark, J.M. (1995) Cloning and sequencing of a cDNA encoding acetylcholinesterase in Colorado potato beetle, Leptinotarsa decemlineata (Say). Insect Biochem. & Mol. Biology 25 (10), 1129-1138.

J. Marshall Clark (ed.), (1995) Molecular Action of Insecticides on Ion Channels. ACS Symposium Series 591, ACS Books, Washington, D.C., pp. 1-356.