Email: kalidas@foodsci.umass.edu
K. Shetty Food Science Dept Website

Ph.D.: University of Idaho
Postdoctoral Training: National Institute of Agro-Biological Research, Japan; University of Guelph, Ontario, Canada

Role of Proline-linked Pentose Phosphate Pathway and Regulation of Secondary Metabolite Synthesis, Stress Response and Development in Plants

Research in my laboratory focuses on molecular physiology of secondary metabolite (phenolic) synthesis in plants. We are particularly interested in studying the role of proline oxidation-linked regulation of the pentose phosphate pathway and how this could regulate metabolite flow to purine and aromatic pathways. Understanding this pathway has implications for producing novel plant metabolites for disease preventive and food preservation applications. At a fundamental level, the understanding of this novel mode of regulation also has implications for understanding plant development (somatic embryogenesis and shoot organogenesis) and stress response (salt, pH, aromatic pollution, and ozone).

Representative publications:

Randhir, R. and Shetty, K. (2006) Elicitation of the proline-linked pentose phosphate pathway metabolite and antioxidant enzyme response by ascorbic acid in dark germinated fava bean sprouts. J. Food Biochemistry, Accepted for publication.

Kwon, Y.I., Apostolidis, E., Labbe, R.G. and Shetty, K. (2006) Inhibition of Staphylooccus aureus by phenolic phytochemicals of selected clonal herb species of Lamiaceae family and likely mode of action through proline oxidation. Food Biotechnology, Accepted for Publication.

Kwon, Y-I., Apostolidis, E. and Shetty, K., (2006) Inhibitory potential of wine and tea against alpha-amylase and alpha-glucosidase for management of hyperglycemia linked to type 2 diabetes. J. Food Biochemistry, Accepted for Publication.

Kwon, Y.I., Apostolidis, E. and Shetty, K. (2006) Evaluation of pepper ( Capsicum annuum ) for management of diabetes and hypertension. J. Food Biochemistry, Accepted for Publication.

Apostolidis, E., Kwon, Y-I. and Shetty, K. (2006) Inhibitory potential of herb, fruit and fungal-enriched cheese against key enzymes linked to type 2 diabetes and hypertension. Innovative Food Science and Emerging Technologies, Accepted for Publication.

Burguieres, E., McCue, P., Kwon, Y-I. and Shetty, K. (2006) Effect of vitamin C and folic acid on seed vigor response and phenolic-linked antioxidant activity. Bioresource Technology, In press.

Apostolidis, E., Kwon, Y-I. and Shetty, K. (2006) Potential of select Yogurts for diabetes and hypertension management. J. Food Biochemistry, Accepted for Publication.

Randhir, R., Vattem, D.A. and Shetty, K. (2006) Antioxidant enzyme response studies in H 2 O 2 -stressed porcine muscle tissue following treatment with fava bean sprout extract and L-DOPA. J. Food Biochemistry, Accepted for Publication.

Horii, A., McCue, P. and Shetty, K. (2006) Enhancement of seed vigor following insecticide and phenolic elicitor treatment. Bioresources Technology, In Press.

Apostolidis, E., Kwon, Y-I. and Shetty, K. (2006) Potential of cranberry-based herbal synergies for diabetes and hypertension management. Asia Pacific J. Clinical Nutrition, In press.

Kwon, Y-I., Jang, H-D. and Shetty, K. (2006) Evaluation of Rhodiola crenulata and Rhodiola rosea for management of Type II diabetes and hypertension. Asia-Pacific J. Clinical Nutrition, In press.

Kwon, Y-I., Apostolidis, E. and Shetty, K. (2006) Anti-Diabetes functionality of Kefir culture-mediated fermented soymilk supplemented with Rhodiola extracts. Food Biotechnology, 20: 13-29.

Kwon, Y-I., Vattem, D.A. and Shetty, K. (2006) Evaluation of clonal herbs of Lamiaceae species for management of diabetes and hypertension. Asia Pacific J. Clinical Nutrition, 15: 107-118.

Vattem, D.A., Jang, H-D., Levin, R.E. and Shetty, K. (2006) Synergism of cranberry phenolics with ellagic acid and rosmarinic acid for antimutagenic and DNA-protection functions. J. Food Biochemistry, 30: 98-116.

Ho, C-H., Lin, Y-T., Labbe, R.G. and Shetty, K. (2006) Inhibition of Helicobacter pylori by phenolic extracts of sprouted peas ( Pisum sativum L. ). J. Food Biochemistry, 30: 21-34.

Lin, Y-T., Kwon, Y-I., Labbe, R.G. and Shetty, K. (2005) Inhibition of Helicobacter pylori and associated urease by oregano and cranberry phytochemical synergies. Applied Environmental Microbiology, 71: 8558-8564.

Lin, Y.T., Labbe, R.G. and Shetty, K. (2005) Inhibition of Vibrio parahaemolyticus in seafood systems using oregano and cranberry synergies and lactic acid. Innovative Food Science and Emerging Technologies, 6: 453-458.

Al-Amier, H.A., Mansour, B.M.M., Toaima, N., Shetty, K. and Craker, L. (2005) Stimulation of high biomass, rosmarinic acid and total phenolics in tissue cultures of Pennyroyal ( Mentha pulegium) in response to Pseudomonas mucidolens . J. Herbs, Spices and Medicinal Plants, 11:13-24.

McCue, P., Lin, Y-T., Labbe, R.G. and Shetty, K. (2005) Characterization of the effect of sprouting and solid-state bioprocessing by dietary fungus on the antinbacterial activity of soybean extracts against Listeria monocytogenes . Food Biotechnology, 19:121-136.

McCue, P., Kwon, Y-I. and Shetty, K. (2005) Anti-amylase , anti-glucosidase and anti-angiotension I converting enzyme potential of select foods. J. Food Biochemistry, 29:278-294.

McCue, P., Kwon, Y-I. and Shetty, K. (2005) Anti-diabetic and anti-hypertensive potential of sprouted and solid-state bioprocessed soybean. Asia Pacific J. Clinical Nutrition, 14: 145-152.

Min, B., Dawson, P.L. and Shetty, K. (2005) Antioxidant and bioactive films to enhance food quality and phytochemical production during ripening. Korean J. Food Sci. Ani. Resour. 25: 60-65.

Vattem, D.A., Lin, Y-T. and Shetty, K. (2005) Enrichment of phenolic antioxidants and anti- Helicobacter pylori properties of cranberry pomace by solid-state bioprocessing. Food Biotechnology, 19: 51-68.

Vattem, D.A., Randhir, R. and Shetty, K. (2005) Cranberry phenolics-mediated elicitation of antioxidant enzyme response in fava bean ( Vicia faba ) sprouts. J. Food Biochemistry, 29: 41-70.

Vattem, D.A., Randhir, R. and Shetty, K. (2005) Cranberry phenolics-mediated antioxidant enzyme response in oxidatively stressed porcine muscle. Process Biochemistry, 40: 2225-2238 .

Lin, Y-T., Vattem, D.A., Labbe, R.G. and Shetty, K. (2005) Enhancement of antioxidant activity and inhibition of Helicobacter pylori by phenolic phytochemical enriched alcoholic beverages. Process Biochemistry, 40: 2059-2065.

Randhir, R., Vattem, D.A. and Shetty, K. (2005) Antioxidant enzyme response studies in H 2 O 2 -stressed porcine muscle tissue following treatment with Oregano phenolic extracts. Process Biochemistry, 40: 2123-2134 .

Vattem, D.A., Lin, Y-T., Ghaedian, R. and Shetty, K. (2005) Cranberry synergies for dietary management of Helicobacter pylori infections. Process Biochemistry, 40: 1583-1592.

McCue, P. and Shetty, K. (2005) Phenolic antioxidant mobilization during yogurt production from soymilk. Process Biochemistry, 40: 1791-1797.

Randhir, R. and Shetty, K. (2005) Developmental stimulation of total phenolics and related antioxidant activity in light and dark-germinated corn by natural elicitors. Process Biochemistry, 40: 1721-1732.

McCue, P. and Shetty, K. (2005) A Model for the Involvement of Lignin Degradation Enzymes in Phenolic Antioxidant Mobilization from Whole Soybean During Solid-state Bioprocessing by Lentinus edodes . Process Biochemistry, 40: 1143-1150.

Chun, S-S., Vattem, D.A., Lin, Y-T. and Shetty, K. (2005) Phenolic antioxidants from clonal oregano ( Origanum vulgare ) with antimicrobial activity against Helicobacter pylori . Process Biochemistry, 40: 809-816.

McCue, P., Vattem, D.A. and Shetty, K. (2004) Inhibitory effect of clonal oregano extracts against porcine pancreatic amylase in vitro. Asia Pacific Journal of Clinical Nutrition, 13: 401-408 .

Correia, R.T.P., McCue, P., Margarida, M.A.M., Macedo, G.R. and Shetty,K (2004) Solid-state bioconversion of guava waste mixed with soy flour using Rhizopus oligosporus to enhance phenolic antioxidants. J. Food Biochemistry, 28: 404-418.

Correia, R.T.P., McCue, P., Vattem, D.A., Margarida, M.A.M., Macedo, G.R. and Shetty,K (2004) Amylase and Helicobacter pylori inhibition by phenolic extracts of pineapple wastes bioprocessed by Rhizopus oligosporus. J. Food Biochemistry, 28: 419-434.

McCue, P., Horii, A. and Shetty, K. (2004) Mobilization of phenolic antioxidants from defatted soybean powders by Lentinus edodes during solid-state fermentation is associated with enhanced production of laccase. Innovative Food Science and Emerging Technologies, 5: 385-392.

Randhir, R., Lin, Y-T. and Shetty, K. (2004) Phenolics, antioxidant and antimicrobial activity in dark germinated fenugreek sprouts in response to peptide and phytochemical elicitors. Asia Pacific Journal of Clinical Nutrition, 13:295-307 .

Lin, Y-T., Labbe, R.G. and Shetty, K. (2004) Inhibition of Listeria monocytogenes in fish and meat systems using oregano and cranberry synergies. Applied and Environmental Microbiology, 70:5672-5678.

McCue, P., Lin Y-T., Labbe, R.G. and Shetty, K. (2004) Sprouting and solid-state bioprocessing by Rhizopus oligosporus increase the in vitro antibacterial activity of aqueous soybean extracts against Helicobacter pylori . Food Biotechnology, 18: 229-249.

Correia, R.T.P., McCue, P., Margarida, M.A.M., Macedo, G.R. and Shetty,K (2004) Production of phenolic antioxidants by solid-state bioconversion of pineapple waste mixed with soy flour using Rhizopus oligosporus . Process Biochemistry, 39:2167-2172.

Vattem, D.A., Lin, Y-T., Labbe, R.G. and Shetty, K. (2004) Antimicrobial activity against select food-borne pathogens by phenolic antioxidants enriched cranberry pomace by solid-state bioprocessing using food-grade fungus Rhizopus oligosporus . Process Biochemistry, 39:1939-1946.

Randhir, R. and Shetty, K. (2004) Microwave-induced stimulation of L-DOPA phenolics and antioxidant activity in fava bean ( Vicia faba ) for Parkinson's diet. Process Biochemistry, 39: 1775-1784.

McCue, P. and Shetty, K. (2004) A role for amylase and peroxidase-linked polymerization in phenolic antioxidant mobilization in dark-germinated soybean and implications for health. Process Biochemistry, 39: 1785-1791.

Randhir, R., Vattem, D. and Shetty, K. (2004) Soild-state bioconversion of fava bean by Rhizopus oligosporus for enrichment of phenolic antioxidants and L-DOPA. Innovative Food Science and Emerging Technologies, 5: 235-244.

McCue, P., and Shetty, K. (2004). Inhibitory effects of rosmarinic acid extracts on porcine pancreatic amylase and implications for health. Asia Pacific Journal of Clinical Nutrition, 13: 101-106.

Vattem, D.A., Lin, Y-T., Labbe, R.G. and Shetty, K. (2004) Phenolic antioxidant mobilization in cranberry pomace by solid-state bioprocessing using food grade fungus Lentinus edodes and effect on antimicrobial activity against select food-borne pathogens. Innovative Food Science and Emerging Technologies, 5: 81-91.

Randhir, R., Lin, Y-T. and Shetty, K. (2004) Stimulation of phenolics, Antioxidant and antimicrobial activities in dark germinated mung bean ( Vigna radiata ) Sprouts in response to peptide and phytochemical elicitors. Process Biochemistry, 39: 637-646 .

Vattem, D.A. and Shetty, K. (2003) Ellagic acid production and phenolic antioxidant activity in cranberry pomace mediated by Lentinus edodes using solid-state system. Process Biochemistry, 39: 367-379 .

McCue, P., Horii, A and Shetty, K. (2003) Solid-state bioconversion of phenolic antioxidants from defatted powdered soybean by Rhizopus oligosprous : Role of carbohydrate cleaving enzymes. J. Food Biochemistry, 27: 499-512.

Vattem, D.A. and Shetty, K. (2003) Acrylamide in food: Probable mechanisms of formation and its reduction. Innovative Food Science and Emerging Technologies, 4: 331-338 .

Shetty, P., Atallah, M.T. and Shetty, K. (2003) Stimulation of total phenolics, L-DOPA and antioxidant activity through proline-linked pentose phosphate pathway in response to proline and its analog in germinating fava beans ( Vicia faba ). Process Biochemistry, 38: 1707-1717.

Seaberg, A., Labbe, R.L. and Shetty, K. (2003) Inhibition of Listeria monocytogenes by elite clonal extracts of oregano ( Origanum vulgare ). Food Biotechnology, 17:129-149.

McCue, P. and Shetty, K. (2003) Role of carbohydrate-cleaving enzymes in phenolic antioxidant mobilization from whole soybean fermented with Rhizopus oligosprorus . Food Biotechnology, 17:27-37.

Randhir, R. and Shetty, K. (2003) Light-mediated fava bean ( Vicia faba ) response to phytochemical and protein elicitors and consequences on nutraceutical enhancement and seed vigor. Process Biochemistry, 38: 945-952.

Shetty, P., Atallah, M.T. and Shetty, K. (2002) Stimulation of total phenolics, L-DOPA and antioxidant activity through proline-linked pentose
phosphate pathway in response to proline and its analog in germinating fava beans (Vicia faba) Process Biochemistry, Submitted.

Randhir, R. and Shetty, K. (2002) Light-mediated fava bean (Vicia faba) response to phytochemical and protein elicitors and consequences on
nutraceutical enhancement and seed vigor. Process Biochemistry, Submitted

Strycharz, S. and Shetty, K. (2002) Response of oregano (Origanum vulgare ) clonal lines to Pseudomonas sp. Z strain and polydye R-478 and
implications for hyperhydricity prevention in tissue culture. Process Biochemistry, Submitted.

Strycharz, S. and Shetty, K. (2002) Effect of Agrobacterium rhizogenes on phenolic content of Mentha pulegium elite clonal line for
phytoremediation applications. Process Biochemistry, Submitted

Shetty, P., Atallah, M.T. and Shetty, K. (2002) Effects of UV treatment on the proline-linked pentose phosphate pathway for phenolics and L-DOPA
synthesis in dark germinated Vicia faba. Process Biochemistry, 37:1285-1295.

Randhir, R., Shetty, P. and Shetty, K.(2002) L-DOPA and total phenolic stimulation in dark geriminated fava bean in response to peptide and
phytochemical elicitors. Process Biochemistry, 37:1247-1256.

McCue, P. and Shetty, K.(2002) A biochemical analysis of mungbean (Vigna radiata) response to microbial polysaccharides and potential
phenolic-enhnacing effects for nutraceutical applications. Food Biotechnology, 16:57-79.

McCue, P. and Shetty, K. (2002) Clonal herbal extracts as elicitors of phenolic synthesis in dark-germinated mungbeans for improving nutritional value
with implications for food safety. J. Food Biochemistry, 26:209-232.

Strycharz, S. and Shetty, K. (2002) Peroxidase activity and phenolic content in elite clonal lines of Mentha pulegium in respense to polymeric dye
R-478 and Agrobacterium rhizogenes. Process Biochemistry, 37:805-812.

Duval, B. and Shetty K. (2001) The stimulation of phenolics and antioxidant activity in pea (Pisum sativum) elicited by genetically transformed anise
root extract. J.Food Biochemistry, 25:361-377.

Kaspera, R., McCue, P. and Shetty, K. (2001) Partial purification of a basic guaiaciol peroxidase from fava bean (Vicia faba L.): Chracterization of
enzyme stability following elicitor treatment. Food Biotechnology, 15: 99-111.

Zheng, Z. and K. Shetty (2000). "Azo dye-mediated regulation of total phenolics and peroxidase activity in thyme (Thymus vulgaris L.) and rosemary (Rosmarinus officinalis L.) clonal lines." J Agric Food Chem 48(3): 932-7.

Zheng, Z. and K. Shetty (2000). "Enhancement of pea (Pisum sativum) seedling vigour and associated phenolic content by extracts of apple pomace fermented with Trichoderma spp." 36(1-2): 79-84.

Zheng, Z. and K. Shetty (2000). "Solid state production of polygalacturonase by Lentinus edodes using fruit processing wastes." 35(8): 825-830.

Zheng, Z. and K. Shetty (2000). "Solid-state bioconversion of phenolics from cranberry pomace and role of Lentinus edodes beta-glucosidase." J Agric Food Chem 48(3): 895-900.

Andarwulan, N., D. Fardiaz, G. A. Wattimena and K. Shetty (1999). "Antioxidant activity associated with lipid and phenolic mobilization during seed germination of Pangium edule Reinw." J Agric Food Chem 47(8): 3158-63.