Handouts and graphs of results, as well as raw data and free software for
data analysis, are available at the
class website.
Operation of the FACStar cell
sorter is also demonstrated.
Microbiology 542, Immunology Laboratory,
is a two-credit course offered
every fall semester. About one third of this course deals with flow
cytometry. Spleen and thymus lymphocytes are prepared from mice,
and the students stain them with fluorescent monoclonal antibodies to over
a dozen distinct lymphocyte surface receptors. The students observe their
data being acquired on the FACScan by a TA (but do not operate the
instrument themselves). Each student spends 4-5 hours at a computer
analyzing the two-color data from their samples, and printing the results.
The printed results are collected in a class book, which each member
then consults before writing a final report on the lymphocyte subpopulations
observed.
Handouts and graphs of results, as well as raw data and free software for
data analysis, are available at the
class website.
Operation of the FACStar cell
sorter is also demonstrated.
By special arrangment when space is available, individuals may take only the flow cytometry portion of Microbiology 542 for one credit.
Independent study, at the undergraduate or graduate levels, can be arranged with Eric Martz to obtain training and experience in flow cytometric methods selected by the student. Such experience could include cell culture and sample preparation as well as hands-on use of the instrument. Methods could include antibody titrations, two-color subpopulation analysis, DNA cell cycle/apoptosis analysis, calcium fluxes, kinetics of binding of antibody or other ligands, cell sorting, or other methods of the student's choice.
A full-semester course in flow cytometric methods is being contemplated but is not yet offered. Contact Eric Martz if interested. emartz@microbio.umass.edu
More information on flow cytometry
is available at the UMass Flow Cytometry Facility Home Page.