Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
|
Disk file
name |
02036h2.gif |
||
|
Student
name |
Bernd Mayer |
||
|
Cell type |
Splenocytes |
Cells in
scatter gate as % of total events |
71% |
SAMPLE PREPARATION
|
Cycle No. |
Ab
Specificity (Marker) |
Fluorochrome
on Ab (if any) |
|
1 |
MHC-2 (rat) |
None |
|
2 |
Polyclonal anti-rat Ig-G (goat) |
FITC |
|
3 |
CD-4
(rat) |
PE |
RESULTS
|
FL1 |
If >1 peak |
Fluorescence
Intensity (FI) |
Control (Blank) |
Corrected
Fluorescence Intensity (CFI)† |
||
|
Peak No.§ |
Peak: %
of (Events in Gate)‡ |
Disk File
number |
Fluorescence
Intensity (FI) |
|||
|
FL1 |
1 |
46 |
5.88 |
26 Autofl. |
4.37 |
1.51 |
|
FL1 |
2 |
53 |
91.4 |
26 Autofl. |
4.37 |
87.0 |
|
FL2 |
1 |
74 |
4.33 |
26 Autofl. |
3.13 |
1.20 |
|
FL2 |
2 |
25 |
83.54 |
26 Autofl. |
3.13 |
80.41 |
|
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|
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|
|
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*Specify
whether FL1 or FL2 for each line in the table.
§Which
peak, the first (from the left), second, ...?
‡ The peak
as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top.
If you have two equal-sized peaks, the peak % of (events in gate) should be 50%
and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the
appropriate control, and subtract it from your sample's FI to get the CFI.