Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
Disk file name  02038
Student name  Nathan K. Gumlaw
Cell type Thymocytes  Cells in scatter gate as % of total events  67%

SAMPLE PREPARATION

Cycle No. Ab Specificity (Marker) Fluorochrome on Ab (if any)
 #1 CD8  Unconjugated 
#2   #A IgG, rat FITC 
#3  CD4  PE

RESULTS

FL1
or
FL2*		 If >1 peak Fluorescence Intensity (FI)
Control (Blank)
 Corrected Fluorescence Intensity (CFI)†
Peak No.§ Peak: % of (Events in Gate)‡ Disk File number Fluorescence Intensity (FI)
 FL1  1 11%   7  026  2.2  5
FL1   2 89%   157  026  2.2  155
FL2   1 1%   2  026  2.6  0
FL2   2 99%   79  026  2.6  76
             
*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.