Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
Disk file name 02046
Student name Allen J Phinney
Cell type Splenocytes Cells in scatter gate as % of total events  62%

SAMPLE PREPARATION

Cycle No. Ab Specificity (Marker) Fluorochrome on Ab (if any)
 1 CD11a Rat IgG, Anti- Mouse  
 2  IgG Polyclonal Goat, Anti-Rat (#A)  FITC-conjugated 
 3 CD4  Rat IgG, Anti-Mouse   PE-conjugated

RESULTS

FL1
or
FL2*		 If >1 peak Fluorescence Intensity (FI)
Control (Blank)
 Corrected Fluorescence Intensity (CFI)†
Peak No.§ Peak: % of (Events in Gate)‡ Disk File number Fluorescence Intensity (FI)
 FL1 1   1%  3  030  3.0  0
2  99%  18  030  3.0  15
FL2  1   75%  4  030  3.1  .9
  2  22%  62  030  3.1 58.9
             
*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?FL1
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.