Immunology Lab 542
FACScan Fluorescence Intensities from Histograms

Disk file name

 01017

Student name

 Craig Gassman

Cell type

 Thymocytes

Cells in scatter gate as % of total events

 65%

SAMPLE PREPARATION

Cycle No.

Ab Specificity (Marker)

Fluorochrome on Ab (if any)

 1

 Thy-1

 FITC

 2

 IgG

 

 3

 CD4

 PE

RESULTS

FL1
or
FL2*

If >1 peak

Fluorescence Intensity (FI)

Control (Blank)

Corrected Fluorescence Intensity (CFI)†

Peak No.§

Peak: % of (Events in Gate)‡

Disk File number

Fluorescence Intensity (FI)

 1

 0.4%

 17

 029

 2.0

 15

 1

 2

 3%

 49

 029

 2.0

 47

 95%

 250

 029

 2.0

 248

 

 

 

 

 

 

 

 2

 7%

 50

 029

 2.0

 48

*Specify whether FL1 or FL2 for each line in the table.

§Which peak, the first (from the left), second, ...?

‡ The peak as a % of (events in gate).

Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.

†Select the appropriate control, and subtract it from your sample's FI to get the CFI.