Immunology Lab 542
FACScan Fluorescence Intensities from Histograms

Disk file name


Student name

 Barbara Meloncourt

Cell type


Cells in scatter gate as % of total events



Cycle No.

Ab Specificity (Marker)

Fluorochrome on Ab (if any)





 Anti IgG rat







If >1 peak

Fluorescence Intensity (FI)

Control (Blank)

Corrected Fluorescence Intensity (CFI)†

Peak No.§

Peak: % of (Events in Gate)‡

Disk File number

Fluorescence Intensity (FI)


































*Specify whether FL1 or FL2 for each line in the table.

§Which peak, the first (from the left), second, ...?

‡ The peak as a % of (events in gate).

Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.

†Select the appropriate control, and subtract it from your sample's FI to get the CFI.