Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
Disk file name  5420334
Student name  Christina DiVincenzo
Cell type  Splenocytes Cells in scatter gate as % of total events  64%

SAMPLE PREPARATION

Cycle No. Ab Specificity (Marker) Fluorochrome on Ab (if any)
 1  CD8  None
 2  IgG Rat  FITC Conjugated
 3  CD4  PE Conjugated

RESULTS

FL1
or
FL2*		 If >1 peak Fluorescence Intensity (FI)
Control (Blank)
 Corrected Fluorescence Intensity (CFI)†
Peak No.§ Peak: % of (Events in Gate)‡ Disk File number Fluorescence Intensity (FI)
FL1 
 1
 96%
 3.5
 026
 2.5
 1.0
 
 2
 4%
 160
 026
 2.5 
 157
 FL2
 1
 63%
 2.41
 025
 2.2
 0.2
 
 2
 37%
 43
 025
 2.2 
 41
             
*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.