Immunology Lab 542
FACScan Fluorescence Intensities from Histograms

Disk file name

 5420307

Student name

Daniel Aiello

Cell type

Splenocytes 

Cells in scatter gate as % of total events

 55

SAMPLE PREPARATION

Cycle No.

Ab Specificity (Marker)

Fluorochrome on Ab (if any)

 1

IgM

PE

 1

MHC II

none

 

 

 

RESULTS

FL1
or
FL2*

If >1 peak

Fluorescence Intensity (FI)

Control (Blank)

Corrected Fluorescence Intensity (CFI)†

Peak No.§

Peak: % of (Events in Gate)‡

Disk File number

Fluorescence Intensity (FI)

FL1

1

49

4.7

026

2.5

2.2

FL1

2

47

79

026

2.5

76

FL2

1

50

4.3

025

2.2

2.1

FL2

2

45

170

025

2.2

168

 

 

 

 

 

 

 

*Specify whether FL1 or FL2 for each line in the table.

§Which peak, the first (from the left), second, ...?

‡ The peak as a % of (events in gate).

Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.

†Select the appropriate control, and subtract it from your sample's FI to get the CFI.