Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
Disk file name  54203038
Student name  Dang Vu
Cell type Thymocytes  Cells in scatter gate as % of total events  62.37%

SAMPLE PREPARATION

Cycle No. Ab Specificity (Marker) Fluorochrome on Ab (if any)
 1
 CD4
none 
 2
 Rat IgG
FITC
 3
 CD8
PE

RESULTS

FL1 
or 
FL2*		 If >1 peak Fluorescence Intensity (FI)
Control (Blank)
 Corrected Fluorescence Intensity (CFI)†
Peak No.§ Peak: % of (Events in Gate)‡ Disk File number Fluorescence Intensity (FI)
 FL1
 1
 4.3
 7.2
54203028
2.0
5.2
 FL1
 2
 95.7
 29.2
 54203028
 2.0
 27.2
 FL2
 1
 10.0
 3.5
 54203028
 2.0
 1.5
 FL2
 2
 90.0
 59.4
54203028
 2.0
 57.4
 
 
 
 
 
 
 
*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.