Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
Disk file name  
Student name  Edda Schulz
Cell type  Thymocytes Cells in scatter gate as % of total events  68%

SAMPLE PREPARATION

Cycle No. Ab Specificity (Marker) Fluorochrome on Ab (if any)
 1  TCR FITC 
 1  CD8 PE 
     

RESULTS

1
FL1
or
FL2*		 If >1 peak Fluorescence Intensity (FI)
Control (Blank)
 Corrected Fluorescence Intensity (CFI)†
Peak No.§ Peak: % of (Events in Gate)‡ Disk File number Fluorescence Intensity (FI)
FL1 1(M4)
 85.44
 9
  026  2.0 7
 FL1 2 (M3)  13.73 70
  026  2.0  68
 FL2 1 (M1)  8.73 5
  026  1.9  3
 Fl2 2 (M2)  90.05 125   026  1.9  123
             
*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.