Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
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Disk file name |
54203051 |
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|
Student name |
Marisa Maddox |
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|
Cell type |
Splenocytes |
Cells in scatter gate as % of total events |
51% |
SAMPLE PREPARATION
|
Cycle No. |
Ab Specificity (Marker) |
Fluorochrome on Ab (if any) |
|
1 |
CD4 |
None |
|
1 |
IgM |
PE |
|
2 |
Goat anti-Rat IgG |
FITC |
RESULTS
|
FL1 |
If >1 peak |
Fluorescence Intensity (FI) |
Control (Blank) |
Corrected Fluorescence Intensity (CFI)† |
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|
Peak No.§ |
Peak: % of (Events in Gate)‡ |
Disk File number |
Fluorescence Intensity (FI) |
|||
|
FL1 |
M1 |
69% |
3.9 |
026 |
2.5 |
1.4 |
|
FL1 |
M2 |
31% |
55 |
026 |
2.5 |
53 |
|
FL2 |
M1 |
47% |
4.5 |
025 |
2.2 |
2.3 |
|
FL2 |
M2 |
52% |
170 |
025 |
2.2 |
170 |
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*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.