Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
|
Disk file name |
W. Matthew Harp, Flow Cytometry Thy 2003 |
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|
Student name |
W. Matthew Harp |
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|
Cell type |
Thymocytes |
Cells in scatter gate as % of total events |
63.06% |
SAMPLE PREPARATION
|
Cycle No. |
Ab Specificity (Marker) |
Fluorochrome on Ab (if any) |
|
1 |
CD45/2 |
None |
|
1 |
IgG, Rat anti-goat |
FITC |
|
2 |
CD4 |
PE-conjugated |
RESULTS
|
FL1 |
If >1 peak |
Fluorescence Intensity (FI) |
Control (Blank) |
Corrected Fluorescence Intensity (CFI)† |
||
|
Peak No.§ |
Peak: % of (Events in Gate)‡ |
Disk File number |
Fluorescence Intensity (FI) |
|||
|
1 |
1 |
2.98 |
2.36 |
029 |
2.2 |
.14 |
|
1 |
1 |
91.98 |
10.18 |
029 |
2.2 |
7.98 |
|
1 |
1 |
5.51 |
201.74 |
029 |
2.2 |
199.54 |
|
2 |
1 |
3.94 |
2.51 |
029 |
2.0 |
.51 |
|
2 |
1 |
6.93 |
5.49 |
029 |
2.0 |
3.49 |
|
2 |
1 |
90.57 |
37.53 |
029 |
2.0 |
35.53 |
*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.