Immunology Lab 542
FACScan Fluorescence Intensities from Histograms

Disk file name

 54203053

Student name

 Maura D. St. John

Cell type

 Splenocytes

Cells in scatter gate as % of total events

59% 

SAMPLE PREPARATION

Cycle No.

Ab Specificity (Marker)

Fluorochrome on Ab (if any)

 1

 IgM

 PE-conjugated

 1

 MHC-1

 None

 2

IgG

 FITC-conjugated

RESULTS

FL1
or
FL2*

If >1 peak

Fluorescence Intensity (FI)

Control (Blank)

Corrected Fluorescence Intensity (CFI)

Peak No.§

Peak: % of (Events in Gate)

Disk File number

Fluorescence Intensity (FI)

 FL1

----- 

           ----------

 184.3

 026

 2.5

 180

 FL2

 1

40% 

 3.9

 026

 2.2 

 1.7

 FL2

 2

 49%

 132.2

 026

 2.2

 130

 

 

 

 

 

 

 

 

 

 

 

 

 

 

*Specify whether FL1 or FL2 for each line in the table.

§Which peak, the first (from the left), second, ...?

The peak as a % of (events in gate).

Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.

†Select the appropriate control, and subtract it from your sample's FI to get the CFI.