Immunology Lab 542
FACScan Fluorescence Intensities from Histograms

Disk file name

 54203054

Student name

 Mulualem E. Tilahun

Cell type

 Thymocyte

Cells in scatter gate as % of total events

 60%

SAMPLE PREPARATION

Cycle No.

Ab Specificity (Marker)

Fluorochrome on Ab (if any)

 1

 Anti CD4

 None-Conjugated

 2

 Anti IgG Rat

 FITC-Conjugated

 3

 Anti CD8

 PE-Conjuated

RESULTS

FL1
or
FL2*

If >1 peak

Fluorescence Intensity (FI)

Control (Blank)

Corrected Fluorescence Intensity (CFI)†

Peak No.§

Peak: % of (Events in Gate)‡

Disk File number

Fluorescence Intensity (FI)

 Fl 1

 1

 1.5%

 4.0

 54203029

 2.0

 2.0

 

 2

 98.5%

 41

 54203029

 2.0

 39

FL2 

 1

 8%

 3.2

 54203029

 2.0

 1.2

 

 2

 92%

 88

 54203029

 2.0

 86

 

 

 

 

 

 

 

*Specify whether FL1 or FL2 for each line in the table.

§Which peak, the first (from the left), second, ...?

‡ The peak as a % of (events in gate).

Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.

†Select the appropriate control, and subtract it from your sample's FI to get the CFI.