Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
Disk file name  54203055
Student name  Nicholas T Charlton
Cell type  Thymocyte Cells in scatter gate as % of total events  66%

SAMPLE PREPARATION

Cycle No. Ab Specificity (Marker) Fluorochrome on Ab (if any)
 1  MHC Class II (rat)  none
 2  IgG, rat FITC-conjugated
 3  CD4 PE-conjugated

RESULTS

FL1
or
FL2*		 If >1 peak Fluorescence Intensity (FI)
Control (Blank)
 Corrected Fluorescence Intensity (CFI)†
Peak No.§ Peak: % of (Events in Gate)‡ Disk File number Fluorescence Intensity (FI)
 FL 1  1  100% 4  029  2.0  2
 FL 2  1 0%  1  029  2.0  0
FL2 2 5%  6  029  2.0  4
FL 2 3  95%  24  029  2.0  22
             
*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.