Immunology Lab 542
FACScan Fluorescence Intensities from Histograms

Disk file name

 54203056

Student name

Nuredin Adem

Cell type

 Thymocytes

Cells in scatter gate as % of total events

 64 %

SAMPLE PREPARATION

Cycle No.

Ab Specificity (Marker)

Fluorochrome on Ab (if any)

 1

Anti MHC I

None

2

Anti IgG rat

FITC

3

CD 8

PE RED

RESULTS

FL1
or
FL2*

If >1 peak

Fluorescence Intensity (FI)

Control (Blank)

Corrected Fluorescence Intensity (CFI)†

Peak No.§

Peak: % of (Events in Gate)‡

Disk File number

Fluorescence Intensity (FI)

FL 1 

1

0.4 %

14

029

2.0

12

 

2

99.6 %

209

029

2.0

207

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

*Specify whether FL1 or FL2 for each line in the table.

§Which peak, the first (from the left), second, ...?

‡ The peak as a % of (events in gate).

Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.

†Select the appropriate control, and subtract it from your sample's FI to get the CFI.