Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
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Disk file name |
03057 |
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Student name |
Rachael Pollina |
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Cell type |
Splenocytes |
Cells in scatter gate as % of total events |
64 % |
SAMPLE PREPARATION
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Cycle No. |
Ab Specificity (Marker) |
Fluorochrome on Ab (if any) |
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1 |
CD45/2 |
none |
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1 |
IgM |
PE |
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2 |
IgG |
FITC |
RESULTS
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FL1 |
If >1 peak |
Fluorescence Intensity (FI) |
Control (Blank) |
Corrected Fluorescence Intensity (CFI)† |
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Peak No.§ |
Peak: % of (Events in Gate)‡ |
Disk File number |
Fluorescence Intensity (FI) |
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Fl 1 |
1 |
100 % |
12 |
26 |
2.5 |
10 |
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Fl 2 |
1 |
52 % |
4 |
25 |
2.2 |
2 |
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Fl 2 |
2 |
45 % |
159 |
25 |
2.2 |
157 |
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*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.