Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
Disk file name  03061 Data Used From 01009 (2001)
Student name  Scott Yarosh
Cell type  Splenocytes Cells in scatter gate as % of total events  74%

SAMPLE PREPARATION

Cycle No. Ab Specificity (Marker) Fluorochrome on Ab (if any)
1 IgM PE 
1 CD18  
2 IgG FITC

RESULTS

FL1
or
FL2*		 If >1 peak Fluorescence Intensity (FI)
Control (Blank)
 Corrected Fluorescence Intensity (CFI)†
Peak No.§ Peak: % of (Events in Gate)‡ Disk File number From 2001 Fluorescence Intensity (FI) From 2001
1   1 M2  6%  2.3  002  3.6  -1.3
 1  2 M1  94%  22  002  3.6  19
 2  1 M2  37%  4.7  002  2.8  1.9
 2  2 M1  63%  346  002  2.8  343
             
*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.