Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
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Disk file name |
5403062cfi.html |
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Student name |
Tamara Lawson |
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Cell type |
Splenocytes |
Cells in scatter gate as % of total events |
62% |
SAMPLE PREPARATION
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Cycle No. |
Ab Specificity (Marker) |
Fluorochrome on Ab (if any) |
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1 |
Rat anti- CD11a |
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1 |
Rat anti-IgM |
PE |
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2 |
Goat anti-rat |
FITC |
RESULTS
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FL1 |
If >1 peak |
Fluorescence Intensity (FI) |
Control (Blank) |
Corrected Fluorescence Intensity (CFI)† |
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Peak No.§ |
Peak: % of (Events in Gate)‡ |
Disk File number |
Fluorescence Intensity (FI) |
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FL1 |
M1 |
99 |
20 |
026 |
2.5 |
18 |
|
FL2 |
M2 |
0.51 |
2.4 |
026 |
2.5 |
-0.1 |
|
FL1 |
M1 |
49 |
4.1 |
026 |
2.2 |
1.9 |
|
FL2 |
M2 |
51 |
190 |
026 |
2.2 |
190 |
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*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.