Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
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Disk file name |
99065h.gif |
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Student name |
Randy Milano |
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Cell type |
Splenocytes |
Cells in scatter gate as % of total events |
71% |
SAMPLE PREPARATION
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Cycle No. |
Ab Specificity (Marker) |
Fluorochrome on Ab (if any) |
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1 |
Thy-1 |
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2 |
IgG (rat) |
FITC-Conjugated |
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3 |
CD-8 |
PE-Conjugate |
RESULTS
|
FL1 |
If >1 peak |
Fluorescence Intensity (FI) |
Control (Blank) |
Corrected Fluorescence Intensity (CFI)† |
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Peak No. |
Peak: % of (Events in Gate)‡ |
Disk File number |
Fluorescence Intensity (FI) |
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|
FL1 |
1 |
61% |
8.4 |
99027 |
4.4 |
3.9 |
|
FL1 |
2 |
38% |
72 |
99027 |
4.4 |
68 |
|
FL2 |
1 |
87% |
5.9 |
99026 |
3.1 |
2.8 |
|
FL2 |
2 |
12% |
18 |
99026 |
3.1 |
15 |
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*Specify whether FL1 or FL2 for each line in the table.
Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.