Single Molecule/ Live Cell Imaging Facility
Welcome to our Imaging Facilities!!
Live Cell Imaging: Yokagawa Spinning Disc Confocal on a Nikon Ti system running Metamorph imaging software
Image shows a mammalian cell in early anaphase. The cell is expressing mCherry Tubulin which labels microtubules red and GFP-TPX2, a microtubule associated protein that binds to spindle fibers (yellow = green and red). At the lower left is a cell that is in telophase. the incipient daughter cells are connected by a midbody, a bundle of microtubules. TPX2 does not label the middle region of the midbody, only the flanking microtubules. 100x
Single Molecule Imaging: Custom-built PALM/TIRF system on a Nikon Ti system running Elements imaging software
Image shows a super resolution of microtubules taken with the new PALM system.
Overview of scope components
Spinning Disc confocal system
• Laser lines available: 488nm and 561nm suitable for imaging GFP, RFP, mcherry, and Cy3 conjugated antibodies..
• Mercury arc lamp available for wide field epi fluorescence imaging
• Dichroic filers available:
1. CFP
2. GFP
3. RFP or Cy3 conjugated antibodies
4. DAPI for nuclear staining
PALM/STORM/TIRF system
• Laser lines available: 405nm, 488nm, 514nm, 561nm, 647nm
• Dual view optics enables simultaneous imaging of two fluorophores
• AOTF allows conventional time lapse and triggered acquisitions
• Optics available to obtain 3D super resolution (STORM/PALM)
• Scheduled appointments for facility trained and approved users only
• Please contact Prof. Patricia Wadsworth (patw@bio.umass.edu) by email to schedule training and appointments for both systems
• Scheduling Calendar: http://calcium.physics.umass.edu/cgi-bin/RossLab.pl
Biology Department Video
