Single Molecule/ Live Cell Imaging Facility
Scheduled appointments for facility trained and approved users only. Make reservations using this Reservation System.
Please contact Prof. Patricia Wadsworth (patw@bio.umass.edu) by email to schedule training and appointments for both systems.
Live Cell Imaging: Yokagawa Spinning Disc Confocal on a Nikon Ti system running Metamorph imaging software

Image shows a mammalian cell in early anaphase. The cell is expressing mCherry Tubulin which labels microtubules red and GFP-TPX2, a microtubule associated protein that binds to spindle fibers (yellow = green and red). At the lower left is a cell that is in telophase. the incipient daughter cells are connected by a midbody, a bundle of microtubules. TPX2 does not label the middle region of the midbody, only the flanking microtubules. 100x
Spinning Disc confocal system technical details
- Laser lines available: 488nm and 561nm suitable for imaging GFP, RFP, mcherry, and Cy3 conjugated antibodies..
- Mercury arc lamp available for wide field epi fluorescence imaging
- Dichroic filers available:
- CFP
- GFP
- RFP or Cy3 conjugated antibodies
- DAPI for nuclear staining
Single Molecule Imaging: Custom-built PALM/TIRF system on a Nikon Ti system running Elements imaging software

Image shows a super resolution of microtubules taken with the new PALM system.
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Current News
- About
- Undergraduate
- Advising
- Major Requirements
- Major Requirements Checklist (PDF)
- Recommended Course Sequences
- Courses Approved as Major Electives
- Declaring a Biology Major
- Integrative Experience
- Minor Requirements
- Departmental Honors
- Study Abroad
- Course Catalog
- Course Offerings 2021-22
- Learning Goals
- Research Opportunities
- Awards & Scholarships
- Residential Programs
- Undergrad Handbook (PDF)
- Petitions and Forms
- 2022 Senior Celebration
- Majors Week
- Graduate
- Faculty
- Research
- Facilities
- Alumni
- Make a Gift…