Stem cross sections of grass mutants more easily converted to biofuels




Histochemical analysis of lignin in stem. Cross sections of stems at the first internode of developmentally equivalent plants were dissected when the flag leaf was 4 cm below the spike. (A,D) wildtype, (B,E) amiR-cad1, and (C,F) amiR-comt4. Wiesner reagents stain lignified tissue in a concentration-indicative manner; heavily lignified tissue stains dark red, while areas with less lignin stain orange-yellow (A,B,C). The Mäule reagent stains S lignin; a shift from red to brown-yellow is representative of a decrease in S lignin (D,E,F).