Using zebrafish to understand the developmental origins of disease

Project Description : 

The Timme-Laragy lab uses the zebrafish and cell culture to understand how embryonic exposure to pollutants affects embryonic development and later-life health outcomes. We have ongoing projects examining the effects of phthalates, perfluorinated compounds, and PCBs on pancreas and liver development, and are investigating toxicant effects on later-life metabolic diseases. We anchor these morphometric and metabolic outcomes with experiments probing the antioxidant defense system and the Nrf2 signaling pathway. We are looking to fill two positions interested in joining the research team for the next 2-3 years. Students will gain skills in zebrafish care, embryo morphology and development, screening of transgenic embryos, genotyping, and toxicology.

Duties and Responsibilities:
- Conduct weekly fish room chores, including fish feeding and fish breeding.
- Maintain and feed live brine shrimp culture to larval, juvenile, and adult zebrafish.
- Screen fish embryos with microscopy for transgenics and mutations.
- Maintain and clean fish tanks, dishes, counters, and floors.
- Conduct experiments on the toxicity of chemicals to fish embryos using established protocols.
- Contribute to data collection for publications, presentations, and reports.
- Attend weekly lab meetings.

Student Ranks Applicable: 

Hours Per Week: 

Paid: 

Credit Hours: 

Honors Thesis: 

Name of Lab: 

The Timme-Laragy Lab

Contact Person: 

Dr. Timme-Laragy

Contact E-mail: 

aliciat@schoolph.umass.edu

Lab website: 

https://www.umass.edu/sphhs/person/faculty/alicia-r-timme-laragy