Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
Disk file name  542-02-039
Student name  Tola Plong
Cell type  Spl Cells in scatter gate as % of total events  65%

SAMPLE PREPARATION

Cycle No. Ab Specificity (Marker) Fluorochrome on Ab (if any)
 1  CD8-Unconjugated rat anti mouse monoclonal Ab  
 2  FITC- Conjugated goat anti-rat IgG  FITC-Conjugated
 3  CD4-PE- Conjugated anti mouse mono  PE-Conjugated

RESULTS

FL1
or
FL2*		 If >1 peak Fluorescence Intensity (FI)
Control (Blank)
 Corrected Fluorescence Intensity (CFI)†
Peak No.§ Peak: % of (Events in Gate)‡ Disk File number Fluorescence Intensity (FI)
FL1   1  92%  4.7  54202030  3.0  1.7
 FL1  2  8%  147  54202030  3.0  144.
 FL2 1   76%  4.7  54202030  3.1  1.6
 FL2  2  23%  132  54202030  3.1  129.
             
*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.