Immunology Lab 542
FACScan Fluorescence Intensities from Histograms
Disk file name  54203039
Student name  Diana Moore
Cell type  Splenocytes Cells in scatter gate as % of total events  68%

SAMPLE PREPARATION

Cycle No. Ab Specificity (Marker) Fluorochrome on Ab (if any)
 1  anti-Thy-1  none
 1  anti-IgM  PE
 2  anti-IgG  FITC

RESULTS

FL1
or
FL2*		 If >1 peak Fluorescence Intensity (FI)
Control (Blank)
 Corrected Fluorescence Intensity (CFI)†
Peak No.§ Peak: % of (Events in Gate)‡ Disk File number Fluorescence Intensity (FI)
 FL1  1  60%  6  026  2.5  3.5
   2   40%  180  026  2.5  178
 FL2 1  47%  3.5  025  2.2  1.3
  2  53%  157  025  2.2  155
             
*Specify whether FL1 or FL2 for each line in the table.
§Which peak, the first (from the left), second, ...?
‡ The peak as a % of (events in gate).
Note that (events in gate) as % of (total events) is entered at the top. If you have two equal-sized peaks, the peak % of (events in gate) should be 50% and 50%, regardless of what % of the (total events) were in the scatter gate.
†Select the appropriate control, and subtract it from your sample's FI to get the CFI.