PPT Slide
Gregarines were introduced, and ASET v.2.00 (Eric Karplus; Science Wares) was used with the non-invasive vibrating probe (Alan M. Shipley; Applicable Electronics) to detect the pH gradient, while MGI VideoWave v.1.0c (MGI Software Corp.) was used to record the movement at every centimeter. Frames were extracted from the movies with AVIQuick v.1.0 (Paul A. Roberts), and ImageJ v1.16f (Wayne Rasband; NIH) was used to find edges and overlap frames. The resulting frames were then analyzed with UmassJJa v1.0, developed by one of the authors (Y.X.).
Cysts and spores were collected from the B. germanica feces (Hoshide et al., 1993) with some slight modifications. Cysts were placed on coverslips and incubated at 27ºC in a Petri dish moist chamber. Extruded spores, Fig. 3, were removed from the coverslip by suspending them in saline and using a pipet to transfer them into Eppendorf tubes. Pictures in Fig. 4 were taken using Kodak Digital Science Microscopy Documentation System 120 (Eastman Kodak Company) with Paint Shop Pro v. 5.01 (Jasc Software, Inc.).