Electrode Construction

Sutter Flaming Brown Puller using glass capillary tubes from 
World Precision Instruments (WPI Item# TW150-4 Thin Wall No Filament, with 1.12 and 1.5 mm. inner and outer diameters, respectively)
'TRODE
TYPE		 SHAPE TIP
DIAM.		 'TRODE
FILL		 LIX LIX
COL.
LEN.
Ca2+
B
2µm
 100 mM CaCl2
FLuka
Ca2+ Ionophore I
Cocktail A
 15-20µm
K+ 
 		  A 
 		 2µm 
 		 100mM KCL 
 		 Fluka 
K+ Ionophore I 
Cocktail B		 180µm 
 
H+ 
 		 B 
 		 2µm 
 		 50mM KCl & 
50mM KH2PO4		 Fluka 
H+ Ionophore I 
Cocktail B		 15-25µm 
 
CO32- 
 		  B*  
 		 2µm 
 		 10mM NaHCO3 in 
150mM NaCl		 WPI # IE-310 
 		 20µm 
 

Last Update: 10/08/98

compiled by E. Faszewski
and J.G. Kunkel