Email: ppomposi@microbio.umass.edu
P.Pomposiello's Microbiology website

Ph.D.in Biology, 1997, University of Michigan.
Harvard University, Dept of Cancer Cell Biology, Post Doctoral Fellow, 1997

Oxidative Stress using Bacterial Models

Our laboratory studies oxidative stress using bacterial models. We are particularly interested in global gene regulation in response to oxidative damage, and we use genetic and genomic approaches to understand antioxidant functions. Current projects include the whole-genome transcriptional profiling of E. coli's response to oxidative damage using gene arrays and the characterization of novel genes induced by oxidative stress and with possible roles in the repair of oxidative damage.

Representative publications:

P. J. Pomposiello, A. Koutsolioutsou, D. Carrasco and B. Demple. SoxS-mediated regulation and genetic analysis of the yggX gene of Escherichia coli. (2003) Journal of Bacteriology, 185 :6624-6632.

P.J. Pomposiello and B. Demple. Global Responses to Free Radical Stress. (2002) Advances in Microbial Physiology. 46 :319-341.

P.J. Pomposiello, M.E.J. Bennik and B. Demple. Genome-wide transcriptional profiling of the E. coli responses to superoxide and Sodium Salicylate. (2001) Journal of Bacteriology. 183 :3890-3902.

P.J. Pomposiello and B. Demple. Global Responses to Free Radical Stress. Advances in Microbial Physiology. Vol. 46, in press.

P.J. Pomposiello and B. Demple. Redox-Operated Genetic Switches: the SoxR and OxyR Transcription Factors. (2001) Trends in Biotechnology. 19:109-114.

P.J. Pomposiello, M.E.J. Bennik and B. Demple. Genome-wide transcriptional profiling of the E. coli responses to superoxide and Sodium Salicylate. (2001) Journal of Bacteriology. 183:3890-3902.

M.E.J. Bennik, PJ. Pomposiello, D.Thorne and B.Demple. Defining a rob Regulon in Escherichia coli Using Transposon Mutagenesis. (2000) Journal of Bacteriology. 182:3794-3801.