Curriculum Vitae: Amy (Moeckel) Regish
Physiologist
Education
2001: M.S. University of Massachusetts, Amherst (Aquatic Toxicology)
1988: B.S. University of Vermont,
Burlington (Animal Sciences)
Professional Experience
8/99-Present: Physiologist S.O. Conte Anadromous Fish Research Center, Turners Falls, MA. Supervisor, Dr. Stephen McCormick
Primary
responsibilities include: maintaining and troubleshooting an
in-stream PIT tag reading system, collection and analysis of data,
preparation of data for presentations and written reports, laboratory
and field sampling of fish blood and tissues, assay development and
validation, plasma hormone analysis (cortisol, T3, T4), plasma ion
analysis, immunocytochemistry, and field collection of juvenile salmon
and adult shad. In addition, I contribute to lab safety
procedures, equipment upkeep, maintaining inventory, training new lab
members, and maintenance and care of laboratory fish.
8/99-3/00: Research Assistant
S.O. Conte Anadromous Fish Research Center, Turners Falls,
MA.
Performed hormone analysis
(ELISA and RIA formats),
plasma ion analysis, histology, microscopy, immunocytochemistry, data
collection, processing, and analysis. This work was primarily
with lab reared salmon, but also involves field sampling using
electro-fishing techniques and PIT tag technology.
9/95-7/99: Research Assistant
University of Massachusetts, Amherst, MA
Primary duty was to conduct
physiological research on
fish species employing fish sampling, molecular biology, toxicological
methods, biochemical techniques, in situ hybridization,
spectrophotometry, and microscopy. In addition, I was
responsible for training several undergraduates in laboratory safety
and basic research methods.
2/94-8/95: Research Technician
III Laboratory of Dr. Edwin G. Krebs, University
of
Washington, Department of Biochemistry and Pharmacology.
Seattle, WA 98195
Primary research project was
the cloning and expression of MAPK kinase
constructs, in SF-9 insect cells, purification of the expressed
proteins, characterization of protein activity and interactions, data
analysis, and presentation. I worked independently and
reported weekly to supervisors for discussion of progress, and future
direction.
7/91-1/94: Research Technician II
Laboratory of Dr. Edwin G. Krebs, University of Washington, Department
of Biochemistry and Pharmacology. Seattle, WA
Primary responsibilities
included independent design and performance of
experimental procedures to evaluate various aspects of cell signaling
related to the MAP kinase cascade. Also provided
collaborative efforts and technical support for several post-doctoral
researchers studying various aspects of MAP kinase cell signaling
cascade. This involved many protein and enzyme analysis
techniques, general lab upkeep, supply maintenance and ordering,
submission of written progress reports, and contributions to technical
writings and journal submissions. (promoted 1/94 to research technician
III).
10/89-2/91: Senior Research
Technician Repligen Corporation, Protein
Therapeutics
Group. One
Kendall Square, Cambridge, MA
Primary duty was to develop
and employ a purification
scheme for a recombinant HTLV1 nuclear regulatory protein,
REX. Once purified, REX was characterized and activity assays
were developed. Monthly progress reports and data
presentations were required.
10/88-2/91: Research
Technician
Repligen Corporation, Protein Therapeutics Group. One
Kendall Square, Cambridge, MA
Worked in a multi group
collaborative manner to conduct
biochemical research, developing purification schemes for various
proteins and their characterization for use in pharmaceutical
testing. (Promoted to senior research technician 10/89).
9/84-9/88: Work
Study Student
University of Vermont, Burlington, Vermont. Department of
Biochemistry
Supported research efforts of graduate students, senior researchers with varying duties, including monoclonal antibody generation, lab maintenance, animal care and handling, and independent research.
Independent Study
10/85-6/86: University of Vermont,
Burlington. Department of
Biochemistry
Evaluated the effects of Disseminated Intravascular Coagulation (DIC) on the condition of blood clotting factor V in the serum of affected patients, employing protein purification, PAGE-SDS electrophoresis and Western Blotting.
International Activities
1999 Technical assistance provided for infant
melatonin study, University of Tromsø, Tromsø, Norway
Publications
McCormick, S.D., Regish, A.M. and Christensen, A.K. 2009. Distinct freshwater and seawater isoforms
of Na+/K+-ATPase in gill chloride cells of Atlantic salmon. Journal of Experimental Biology 212: 3994-4001.
McCormick, S.D., Regish, A.M., O'Dea, M.F.
and Shrimpton, J.M. 2008. Are we missing a
mineralocorticoid
in teleost fish? Effects of cortisol, deoxycorticosterone and
aldosterone on osmoregulation, gill Na+/K+-ATPase activity and isoform
mRNA levels in Atlantic salmon. General and Comparative Endocrinology 157: 35-40.
McCormick, S.D., O'Dea,
M.F., Moeckel, A.M., Lerner, D.T. and Björnsson, B.Th. 2005. Endocrine
disruption of parr-smolt transformation and seawater tolerance of
Atlantic salmon by 4-nonylphenol and 17β-estradiol. General and
Comparative Endocrinology 142: 280-288
McCormick, S.D., O'Dea,
M.F., Moeckel, A.M., Björnsson, B.Th. 2003. Endocrine and physiological
changes in Atlantic salmon smolts following hatchery release.
Aquaculture 222: 45-57
Campbell, J.S., Seger, R., Jensen, A.M.,
and Krebs, E.G. The Map Kinase Cascade. Recent
Progress in
Hormone Research. Vol. 50, pp. 131-159, 1995.
Ahn, N.G., Campbell, J.S., Seger, R., Jensen, A.M.,
Graves, L.M., and Krebs, E.G.. Metabolic Labeling of Mitogen Activated
Protein Kinase Kinase in A431 Cells Demonstrates Phosphorylation on
Serine and Threonine Residues. Proc. Natl. Acad. Sci. Vol.
90,
pp. 5143-5147, June 1993.
Seger, R., Ahn, N.G., Posada, J., Munar,
E.S.,Jensen, A.M.,Cooper,
J.,Cobb, M.H., and Krebs, E.G..
Purification and
Characterization of Mitogen Activated Protein Kinase Activators from
Epidermal Growth Factor Stimulated A431 Cells. The Journal of
Biological Chemistry. Vol. 267(20), pp.
14373-14381, July
15, 1992.
Seger, R., Seger, D., Lozeman, F.J., Ahn, N.G., Graves, L.M., Campbell,
J.S., Ericsson, L., Harrylock, M., Jensen, A.M.,
and Krebs, E.G. Human T-Cell Mitogen Activated Protein Kinase
Kinases are Related to Yeast Signal Transduction Kinases. The
Journal of Biological Chemistry. Vol. 267(36), pp.
25628-25631, Dec 25, 1992.
Farrington, G.K.,
Lynch, P.,Jensen, A.,
Bonhlein, E., Doten, V, Maione, T., Daly, T., and Rusche, J.
The
Lentivirus Regulatory Proteins REV and REX are Site Specific RNA
Binding Proteins. Adv. Exp. Med. Biol. Vol. 303,
pp. 15-22, 1991
