Failing to Repair Syntelics Leads to Aneuploidy

Spinning disk confocal live-imaging of an S2 cell with the chromosomes in red, and the microtubules in green. In this case, the syntelic attachment (right side) fails to be corrected in a timely manner and both sister chromatids are pulled towards the same pole. This is a dangerous outcome since the resultant daughter cells will be aneuploid.

Error Correction in Action

Spinning disk confocal live-imaging of an S2 cell with the chromosomes in red, and the kinetochores in green (Ndc80-GFP). Note that the syntelic attachment is corrected and bioriented before anaphase onset. We have seen many examples like this one where cells wait for a single chromosome to become bioriented before committing to anaphase.

Mitosis in Drosophila S2 Cells

The Drosophila S2 "supercell" line proceeding through mitosis. The cell is expressing tubulin-mCherry, Ndc80-GFP (kinetochores) and gamma-tubulin-GFP (centrosomes). This live cell was imaged with a spinning disk confocal microscope every 1 minute.