Sample 3-D Vibrating Probe Output
hummer logo c UMass Red Left UMass Red Right hummer logo d

Samples of 3-D Patterns Measured at UMass Amherst


Automated stereo scan of artificial twin proton point sources using the ASET Software of Applicable Electronics and usin R-scripts for direct analysis of the ASET output. The top 4 panels illustrate the steps in analysis of the 3-D data obtained by the ASET software. Panel 1 illustrates the three orthogonal microvolt differences measured at a grid of observation points, uVdif(xyz), plotted on the Y-axis as uVxyz in colors rgb. In panel 2 the expected -pH calculated from the measured voltage at the origin of each grid point measurement is based on the pH callibration curve. In panel 3 the Expected proton concentration at the grid popint origins is calculated by exponentiating the points in panel 2. The proton concentration difference is then calculated in panel 4 from the voltage at the origin, the uV differences, the calibration curve and the estimated dynamic efficiency of the sampling protocol. For protons and chloride the dynamic efficiency for typical 3-D measuring protocols used recently has been indistinguishable from 100%. The bottom 4 panels illustrate the calculated fluxes calculated from the upper panel data and plotted as stereo pairs of 3-D flux vectors (left pair) or total flux (right pair). Gnu-Public R analysis includes library plotting functions scatterplot3d() and grid plotting function persp(). Triple scan of double H Source
An older automatic triple scan of artificial twin proton point sources with a vibrating proton selective microelectrode, NVPH. The data was collected using the VPW software from Science Wares using a Direct Coupled Ion Probe Amplifier from Applicable Electronics. The data is plotted using Gnu-Public QuikGrid Ver. 3.3 (formerly SURFACE.exe) from John Coulthard.
Triple scan of double H Source Double proton source

Stereo scan of a twin proton source with a proton selective microelectrode 48 hours prior to the above triple scan. This particular electrode survived for 6 days, faithfully recording data from the same pair of artificial sources. Data was prepared to plot using the MAGE software available from FASEB.

Mage Stereo View of double H Source

Portions of adjacent follicles from a Periplaneta americana ovariole with a proton ion selective electrode poised at a sampling site. Data from such a follicle is displayed below.

Periplaneta americana follicle

Proton flux (red vectors) eminating from the anterior end of a Periplaneta americana follicle (Kunkel & Faszewski, 1995). The protons exit from the follicle epithelium (green outline) seen to encase the oocyte (yellow outline). This is a stereo pair produced with MAGE. (blue marks the path of the scanning electrode). Data was collected with a Direct Coupled Amplifier using the 3DVIS software developed by Joe Kunkel at the NVP Facility at the MBL, Woods Hole, MA.

kinemage of Periplaneta americana follicle

View the kin file

If you have questions about visiting or using the University of Massachusetts Vibrating Probe Facility, email its PI, Joe Kunkel


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Page maintained by Joe Kunkel, joe@bio.umass.edu. Copyright(c) 1995. Created: 95/10/28 Updated: 98/05/21